Construct: ORF TRCN0000466286

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002564.1_s317c1
Derived from:
ccsbBroadEn_15521
DNA Barcode:
TGCTGCTAACGTGGTAAGAACGAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SERPINF1 (5176)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466286
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5176 SERPINF1 serpin family F member 1 NM_001329903.1 99.7% 99.5% 215C>T;395C>G;963T>C
2 human 5176 SERPINF1 serpin family F member 1 NM_002615.7 99.7% 99.5% 215C>T;395C>G;963T>C
3 human 5176 SERPINF1 serpin family F member 1 NM_001329904.1 55.1% 55.2% 0_1ins561;402T>C
4 human 5176 SERPINF1 serpin family F member 1 NM_001329905.1 55.1% 55.2% 0_1ins561;402T>C
5 mouse 20317 Serpinf1 serine (or cysteine) peptid... NM_011340.3 83.7% 85.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1320
ORF length:
1254
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca ggccctggtg ctactcctct gcattggagc cctcctcggg cacagcagct
121gccagaaccc tgccagcccc ccggaggagg gctccccaga ccccgacagc acaggggcgc
181tggtggagga ggaggatcct ttcttcaaag tccccgtgaa caagctggca gcggctgtct
241ccaacttcgg ctatgacctg taccgggtgc gatccagcat gagccccacg accaacgtgc
301tcctgtctcc tctcagtgtg gccacggccc tctcggccct ctcgctggga gcggagcagc
361gaacagaatc catcattcac cgggctctct actatgactt gatcagcagc ccagacatcc
421atggtaccta taaggagctc cttgacacgg tcactgcccg ccagaagaac ctcaagagtg
481cctcccggat cgtctttgag aagaagctgc gcataaaatc cagctttgtg gcacctctgg
541aaaagtcata tgggaccagg cccagagtcc tgacgggcaa ccctcgcttg gacctgcaag
601agatcaacaa ctgggtgcag gcgcagatga aagggaagct cgccaggtcc acaaaggaaa
661ttcccgatga gatcagcatt ctccttctcg gtgtggcgca cttcaagggg cagtgggtaa
721caaagtttga ctccagaaag acttccctcg aggatttcta cttggatgaa gagaggaccg
781tgagggtccc catgatgtcg gaccctaagg ctgttttacg ctatggcttg gattcagatc
841tcagctgcaa gattgcccag ctgcccttga ccggaagcat gagtatcatc ttcttcctgc
901ccctgaaagt gacccagaat ttgaccttga tagaggagag cctcaccTCC GAGTTCATTC
961ATGACATAGA CCGAGAACTG AAGACCGTGC AGGCGGTCCT CACTGTCCCC AAGCTGAAGC
1021TGAGTTACGA AGGCGAAGTC ACCAAGTCCC TGCAGGAGAT GAAGCTGCAA TCCTTGTTTG
1081ATTCACCAGA CTTTAGCAAG ATCACAGGCA AACCCATCAA GCTGACTCAG GTGGAACACC
1141GGGCTGGCTT TGAGTGGAAC GAGGATGGGG CGGGAACCAC CCCCAGCCCA GGGCTGCAGC
1201CTGCCCACCT CACCTTCCCG CTGGACTATC ACCTTAACCA GCCTTTCATC TTCGTACTGA
1261GGGACACAGA CACAGGGGCC CTTCTCTTCA TTGGCAAGAT TCTGGACCCC AGGGGCCCCT
1321ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1381GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1441TTTATATATC TTGTGGAAAG GACGATGCTG CTAACGTGGT AAGAACGAGA CGCGTTAAGT
1501Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)