Construct: ORF TRCN0000466291
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013857.1_s317c1
- Derived from:
- ccsbBroadEn_15399
- DNA Barcode:
- ACTAATTGATGGCGTAAGAATCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYNC1I1 (1780)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466291
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_001278421.1 | 99.8% | 99.8% | 1431C>A;1491G>T;1734G>A |
| 2 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_001135557.1 | 97.1% | 97.1% | (many diffs) |
| 3 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_004411.4 | 96.7% | 96.7% | (many diffs) |
| 4 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_001135556.1 | 94.1% | 94.1% | (many diffs) |
| 5 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_001278422.1 | 92.2% | 90.1% | (many diffs) |
| 6 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_001191023.1 | 91.2% | 97.9% | (many diffs) |
| 7 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_001191025.1 | 88.5% | 95.2% | (many diffs) |
| 8 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | XM_006504982.3 | 88.4% | 94.8% | (many diffs) |
| 9 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_001191027.1 | 86.8% | 93.4% | (many diffs) |
| 10 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_010063.4 | 85.8% | 92.2% | (many diffs) |
| 11 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_001191026.1 | 84.1% | 90.5% | (many diffs) |
| 12 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | XM_017321380.1 | 84.1% | 90.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1941
- ORF length:
- 1875
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgacaaaagt gacttaaaag ctgagctaga gcgcaaaaag cagcgcttag 121 cacagataag agaagagaag aaacggaagg aagaggagag gaaaaagaaa gaggctgata 181 tgcagcagaa gaaagaaccc gttcaggacg actctgatct ggatcgcaaa cgacgagaga 241 cagaggcttt gctgcaaagc attggtatct caccggagcc gcctctagtg cagccgctgc 301 attttttaac atgggatacc tgttattttc attatttagt cccaacccct atgtctccct 361 cctcgaaatc agtgagcact cccagtgaag ctggaagcca agactcaggc gatctggggc 421 cattaacaag aagaagactg cataaactgg gcgtgtcaaa ggtcacccaa gtggatttcc 481 tgccaaggga agtagtgtcc tactcaaagg agacccagac tcctcttgcc acgcatcagt 541 ctgaagagga tgaggaagat gaggaaatgg tggaatctaa agttggccag gactcagaac 601 tggaaaatca ggacaaaaaa caggaagtga aggaagcccc tccaagagag ttgacagagg 661 aagaaaaaca gcagatcctt cattcagagg aatttctcat cttttttgac cggacaatac 721 gggtaattga aagagccctg gctgaagatt ccgacatctt ttttgactac agcggccgag 781 agttagagga aaaagatggg gatgttcagg ctggagccaa tctttctttc aatcgtcagt 841 tctatgatga acattggtcc aagcatcgag tggtcacttg tatggactgg tccctccagt 901 accctgagct gatggtggct tcttacaaca acaatgaaga tgctccccat gaaccagatg 961 gagtggcctt ggtttggaac atgaagttta agaaaaccac accagaatac gtcttccact 1021 gtcagtcctc tgtgatgtcg gtctgcttcg cccgtttcca tcctaacttg gtggttggtg 1081 ggacttactc gggccagatt gtcctctggg acaatcgcag tcatcgaagg actccagtgc 1141 agcggacacc cttatcagct gctgcacaca cgcatcccgt gtactgtgta aatgttgttg 1201 ggacccagaa tgctcataac ctcatcactg tctccactga tggcaaaatg tgttcctgga 1261 gcctggacat gctctcaact ccacaggaga gcatggagct ggtgtacaat aagtccaagc 1321 ctgtcgctgt taccggaatg gctttcccaa cgggagacgt caataacttc gtggttggca 1381 gtgaggaagg tacagtctac acggcttgtc gtcatggaag caaagcaggt attggtgagg 1441 tctttgaagg tcaccaaggg ccagtgacag gaattaactg ccacatggca gtgggaccaa 1501 tcgacttttc tcacctgttt gtcacatcat catttgactg gactgtcaaa ctgtgtacca 1561 ccaagcacaa caagccgctc tactcctttg aagacaatgc agactatgtg tacgatgtca 1621 tgtggTCCCC CGTGCATCCT GCGCTTTTTG CCTGCGTGGA CGGGATGGGG CGCTTGGACC 1681 TCTGGAACCT CAACAATGAC ACCGAGGTTC CAACAGCAAG TGTGGCCATT GAGGGGGCAT 1741 CCGCCCTAAA CCGTGTTCGT TGGGCCCAAG CTGGCAAAGA AGTTGCTGTT GGGGACTCAG 1801 AAGGCCGTAT TTGGGTCTAT GACGTTGGAG AGCTTGCAGT TCCCCACAAT GATGAATGGA 1861 CCCGATTTGC CAGGACCCTT GTGGAAATTC GTGCTAACAG AGCTGATAGC GAGGAGGAAG 1921 GCACTGTTGA GTTATCTGCC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1981 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2041 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACTA ATTGATGGCG 2101 TAAGAATCCG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt