Transcript: Mouse XM_017321380.1

PREDICTED: Mus musculus dynein cytoplasmic 1 intermediate chain 1 (Dync1i1), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Dync1i1 (13426)
Length:
2489
CDS:
79..1932

Additional Resources:

NCBI RefSeq record:
XM_017321380.1
NBCI Gene record:
Dync1i1 (13426)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017321380.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000089626 GCTGGTGTATAATAAGTCCAA pLKO.1 1287 CDS 100% 2.640 3.696 N Dync1i1 n/a
2 TRCN0000424148 ATATGAAGCCAGATATGATTG pLKO_005 2279 3UTR 100% 10.800 7.560 N DYNC1I1 n/a
3 TRCN0000089627 CATCTTCTTTGACCGGACAAT pLKO.1 687 CDS 100% 4.950 3.465 N Dync1i1 n/a
4 TRCN0000089624 CGTCAGTTCTACGATGAACAT pLKO.1 823 CDS 100% 4.950 3.465 N Dync1i1 n/a
5 TRCN0000089623 GCTGTATTTAATAGCTGGAAA pLKO.1 2205 3UTR 100% 4.950 3.465 N Dync1i1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017321380.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06111 pDONR223 100% 89.5% 96% None (many diffs) n/a
2 ccsbBroad304_06111 pLX_304 0% 89.5% 96% V5 (many diffs) n/a
3 TRCN0000474666 ACAAAAGTCTGGCCGGTCAGATAC pLX_317 28.5% 89.5% 96% V5 (many diffs) n/a
4 ccsbBroadEn_06110 pDONR223 100% 89.5% 96.1% None (many diffs) n/a
5 ccsbBroad304_06110 pLX_304 0% 89.5% 96.1% V5 (many diffs) n/a
6 TRCN0000474539 GTATGAACGGGGAGATCAGTGTAC pLX_317 26.3% 89.5% 96.1% V5 (many diffs) n/a
7 TRCN0000489183 ACCGTTATATTTCTCCACCACGAA pLX_317 16.4% 89.5% 96.1% V5 (not translated due to prior stop codon) (many diffs) n/a
8 ccsbBroadEn_15399 pDONR223 0% 84.1% 90.5% None (many diffs) n/a
9 ccsbBroad304_15399 pLX_304 0% 84.1% 90.5% V5 (many diffs) n/a
10 TRCN0000466291 ACTAATTGATGGCGTAAGAATCCG pLX_317 17.2% 84.1% 90.5% V5 (many diffs) n/a
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