Construct: ORF TRCN0000466294
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017879.1_s317c1
- Derived from:
- ccsbBroadEn_03766
- DNA Barcode:
- TGCATTCGATCGACAGGTTTTAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDC42SE2 (56990)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466294
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56990 | CDC42SE2 | CDC42 small effector 2 | NM_001038702.1 | 100% | 100% | |
| 2 | human | 56990 | CDC42SE2 | CDC42 small effector 2 | NM_020240.2 | 100% | 100% | |
| 3 | human | 56990 | CDC42SE2 | CDC42 small effector 2 | XM_017009648.2 | 100% | 100% | |
| 4 | human | 56990 | CDC42SE2 | CDC42 small effector 2 | XM_017009649.2 | 100% | 100% | |
| 5 | mouse | 72729 | Cdc42se2 | CDC42 small effector 2 | NM_178626.3 | 90.8% | 100% | (many diffs) |
| 6 | mouse | 72729 | Cdc42se2 | CDC42 small effector 2 | XM_006534320.3 | 90.8% | 100% | (many diffs) |
| 7 | mouse | 72729 | Cdc42se2 | CDC42 small effector 2 | XM_030246369.1 | 90.8% | 100% | (many diffs) |
| 8 | mouse | 72729 | Cdc42se2 | CDC42 small effector 2 | XM_030246370.1 | 90.8% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 345
- ORF length:
- 252
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGAGTGA ATTCTGGTTG TGTTTCAACT 121 GCTGTATTGC AGAACAGCCT CAGCCTAAAA GGCGACGGCG GATTGACAGA AGTATGATTG 181 GAGAGCCCAC AAACTTTGTG CATACAGCTC ATGTTGGATC AGGAGACCTG TTCAGTGGAA 241 TGAATTCAGT TAGCTCCATT CAGAACCAAA TGCAGTCCAA GGGAGGTTAT GGAGGTGGAA 301 TGCCTGCCAA TGTCCAGATG CAGCTCGTGG ATACGAAGGC GGGATTGCCA ACTTTCTTGT 361 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 421 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 481 GAAAGGACGA TGCATTCGAT CGACAGGTTT TAAAACGCGT TAAGTCgaca atcaacctct 541 ggattacaaa atttgtgaaa gatt