Construct: ORF TRCN0000466319
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006387.1_s317c1
- Derived from:
- ccsbBroadEn_06499
- DNA Barcode:
- GCTCTTGCTCTGCGATCATTGGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KPNA2 (3838)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466319
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3838 | KPNA2 | karyopherin subunit alpha 2 | NM_001320611.1 | 99.8% | 99.6% | 470C>T;1152T>C;1359A>C |
| 2 | human | 3838 | KPNA2 | karyopherin subunit alpha 2 | NM_002266.4 | 99.8% | 99.6% | 470C>T;1152T>C;1359A>C |
| 3 | mouse | 16647 | Kpna2 | karyopherin (importin) alpha 2 | NM_010655.3 | 87.3% | 94.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1653
- ORF length:
- 1587
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc caccaacgag aatgctaata caccagctgc ccgtcttcac agattcaaga 121 acaagggaaa agacagtaca gaaatgaggc gtcgcagaat agaggtcaat gtggagctga 181 ggaaagctaa gaaggatgac cagatgctga agaggagaaa tgtaagctca tttcctgatg 241 atgctacttc tccgctgcag gaaaaccgca acaaccaggg cactgtaaat tggtctgttg 301 atgacattgt caaaggcata aatagcagca atgtggaaaa tcagctccaa gctactcaag 361 ctgccaggaa actactttcc agagaaaaac agccccccat agacaacata atccgggctg 421 gtttgattcc gaaatttgtg tccttcttgg gcagaactga ttgtagtccc attcagtttg 481 aatctgcttg ggcactcact aacattgctt ctgggacatc agaacaaacc aaggttgtgg 541 tagatggagg tgccatccca gcattcattt ctctgttggc atctccccat gctcacatca 601 gtgaacaagc tgtctgggct ctaggaaaca ttgcaggtga tggctcagtg ttccgagact 661 tggttattaa gtacggtgca gttgacccac tgttggctct ccttgcagtt cctgatatgt 721 catctttagc atgtggctac ttacgtaatc ttacctggac actttctaat ctttgccgca 781 acaagaatcc tgcacccccg atagatgctg ttgagcagat tcttcctacc ttagttcggc 841 tcctgcatca tgatgatcca gaagtattag cagatacctg ctgggctatt tcctacctta 901 ctgatggtcc aaatgaacga attggcatgg tggtgaaaac aggagttgtg ccccaacttg 961 tgaagcttct aggagcttct gaattgccaa ttgtgactcc tgccctaaga gccataggga 1021 atattgtcac tggtacagat gaacagactc aggttgtgat tgatgcagga gcactcgccg 1081 tctttcccag cctgctcacc aaccccaaaa ctaacattca gaaggaagct acgtggacaa 1141 tgtcaaacat cacagccggc cgccaggacc agatacagca agttgtgaat catggattag 1201 tcccattcct tgtcagcgtt ctctcTAAGG CAGATTTTAA GACACAAAAG GAAGCTGTGT 1261 GGGCCGTGAC CAACTATACC AGTGGTGGAA CAGTTGAACA GATTGTGTAC CTTGTTCACT 1321 GTGGCATAAT AGAACCGTTG ATGAACCTCT TAACTGCAAA AGATACCAAG ATTATTCTGG 1381 TTATCCTGGA TGCCATTTCA AATATCTTTC AGGCTGCTGA GAACCTAGGT GAAACTGAGA 1441 AACTTAGTAT AATGATTGAA GAATGTGGAG GCTTAGACAA AATTGAAGCT CTACAAAACC 1501 ATGAAAATGA GTCTGTGTAT AAGGCTTCGT TAAGCTTAAT TGAGAAGTAT TTCTCTGTAG 1561 AGGAAGAGGA AGATCAAAAC GTTGTACCAG AAACTACCTC TGAAGGCTAC ACTTTCCAAG 1621 TTCAGGATGG GGCTCCTGGG ACCTTTAACT TTTACCCAAC TTTCTTGTAC AAAGTGGTTG 1681 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1741 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGC 1801 TCTTGCTCTG CGATCATTGG TAACGCGTTA AGTCgacaat caacctctgg attacaaaat 1861 ttgtgaaaga tt