Construct: ORF TRCN0000466360
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009372.1_s317c1
- Derived from:
- ccsbBroadEn_06888
- DNA Barcode:
- AACACTTTGTTCCTTAGACAGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPN1 (6184)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466360
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6184 | RPN1 | ribophorin I | NM_002950.4 | 99.8% | 100% | 423G>A;1206C>T |
| 2 | mouse | 103963 | Rpn1 | ribophorin I | NM_133933.4 | 87.7% | 95% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1887
- ORF length:
- 1821
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggcgccagcc gccggcttgt ttctgctcct gttgcttggg acttgggccc 121 cggcgccggg cagcgcctcc tccgaggcac cgccgctgat caatgaggac gtgaagcgca 181 cagtggacct aagcagccac ctggctaagg tgacggccga ggtggtcctg gcgcacctgg 241 gcggcggctc cacgtcccga gctacctctt tcctgctggc tttggagcct gagctcgagg 301 cccggctggc gcacctgggc gtgcaggtaa agggagaaga tgaggaagag aacaatttgg 361 aagtacgtga aaccaaaatt aagggtaaaa gtgggagatt cttcacagtc aagctcccag 421 ttgctcttga tcctggggcc aagatttcag tcattgtgga aacagtctac acccatgtgc 481 ttcatccata tccaacccag atcacccagt cagagaaaca gtttgtggtg tttgagggga 541 accattattt ctactctccc tatccaacga agacacaaac catgcgtgtg aagcttgcct 601 ctcgaaatgt ggagagctac accaagctgg ggaaccccac gcgctctgag gacctactgg 661 attatgggcc tttcagagat gtgcctgcct atagtcagga tacttttaaa gtacattatg 721 agaacaacag ccctttcctg accatcacca gcatgacccg agtcattgaa gtctctcact 781 ggggtaatat tgctgtggaa gaaaatgtgg acttaaagca cacaggagct gtgcttaagg 841 ggcctttctc acgctatgat taccagagac agccagatag tggaatatcc tccatccgtt 901 cttttaagac catccttcct gctgctgccc aggatgttta ttaccgggat gagattggca 961 atgtttctac cagccacctc cttattttgg atgactctgt agagatggaa atccggcctc 1021 gcttccctct ctttggcggg tggaagaccc attacatcgt tggctacaac ctcccaagct 1081 atgagtacct ctataatttg ggtgaccagt atgcactgaa gatgaggttt gtggaccatg 1141 tgtttgatga acaagtgata gattctctga ctgtgaagat catcctgcct gaaggagcca 1201 agaacattga aattgatagt ccctatgaaa tcagccgtgc cccagatgag ctgcactaca 1261 cctatctgga tacatttggc cgccctgtga ttgttgccta caagaaaaat ctggtagaac 1321 agcacattca ggacattgtg gtccactaca cgttcaacaa ggtgctcatg ctgcaggagc 1381 ccctgctggt ggtggcggcc ttctacatcc tgttcttcac cgttatcatc tatgttcggc 1441 tggacttctc catcaccaag gatccagccg cagaagccag gatgaaggta gccTGCATCA 1501 CAGAGCAGGT CTTGACCCTG GTCAACAAGA GAATAGGCCT TTACCGTCAC TTTGACGAGA 1561 CCGTCAATAG GTACAAGCAA TCCCGGGACA TCTCCACCCT CAACAGTGGC AAGAAGAGCC 1621 TGGAGACTGA ACACAAGGCC TTGACCAGTG AGATTGCACT GCTGCAGTCC AGGCTGAAGA 1681 CAGAGGGCTC TGATCTGTGC GACAGAGTGA GCGAAATGCA GAAGCTGGAT GCACAGGTCA 1741 AGGAGCTGGT GCTGAAGTCG GCGGTGGAGG CTGAGCGCCT GGTGGCTGGC AAGCTCAAGA 1801 AAGACACGTA CATTGAGAAT GAGAAGCTCA TCTCAGGAAA GCGCCAGGAG CTGGTCACCA 1861 AGATCGACCA CATCCTGGAT GCCCTGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1921 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1981 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAACACTTT 2041 GTTCCTTAGA CAGTAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2101 aagatt