Construct: ORF TRCN0000466365
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017855.1_s317c1
- Derived from:
- ccsbBroadEn_06027
- DNA Barcode:
- TCGCTTATATTCATGCGACGACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPB2 (1361)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466365
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1361 | CPB2 | carboxypeptidase B2 | NM_001872.5 | 99.7% | 99.7% | 291T>C;663A>G;1040T>C |
| 2 | human | 1361 | CPB2 | carboxypeptidase B2 | XM_017020393.2 | 96% | 94.7% | (many diffs) |
| 3 | human | 1361 | CPB2 | carboxypeptidase B2 | NM_001278541.1 | 91% | 91% | 291T>C;591_592ins111;929T>C |
| 4 | mouse | 56373 | Cpb2 | carboxypeptidase B2 (plasma) | NM_019775.3 | 83.3% | 83.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1335
- ORF length:
- 1269
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gctttgcagc cttgcagtcc ttgtacccat tgttctcttc tgtgagcagc 121 atgtcttcgc gtttcagagt ggccaagttc tagctgctct tcctagaacc tctaggcaag 181 ttcaagttct acagaatctt actacaacat atgagattgt tctctggcag ccggtaacag 241 ctgaccttat tgtgaagaaa aaacaagtcc atttttttgt aaatgcatct gatgtcgaca 301 atgtgaaagc ccatttaaat gtgagcggaa ttccatgcag tgtcttgctg gcagacgtgg 361 aagatcttat tcaacagcag atttccaacg acacagtcag cccccgagcc tccgcatcgt 421 actatgaaca gtatcactca ctaaatgaaa tctattcttg gatagaattt ataactgaga 481 ggcatcctga tatgcttaca aaaatccaca ttggatcctc atttgagaag tacccactct 541 atgttttaaa ggtttctgga aaagaacaag cagccaaaaa tgccatatgg attgactgtg 601 gaatccatgc cagagaatgg atctctcctg ctttctgctt gtggttcata ggccatataa 661 ctcaattcta tgggataata gggcaatata ccaatctcct gaggcttgtg gatttctatg 721 ttatgccggt ggttaatgtg gatggttatg actactcatg gaaaaagaat cgaatgtgga 781 gaaagaaccg ttctttctat gcgaacaatc attgcatcgg aacagacctg aataggaact 841 ttgcttccaa acactggtgt gaggaaggtg catccagttc ctcatgctcg gaaacctact 901 gtggacttta tcctgagtca gaaccagaag tgaaggcagt ggctagtttc ttgagaagaa 961 ataTCAACCA GATTAAAGCA TACATCAGCA TGCATTCATA CTCCCAGCAT ATAGTGTTTC 1021 CATATTCCTA TACACGAAGT AAAAGCAAAG ACCATGAGGA ACTGTCTCTA GTAGCCAGTG 1081 AAGCAGTTCG TGCTATTGAG AAAACTAGTA AAAATACCAG GTATACACAT GGCCATGGCT 1141 CAGAAACCTT ATACCTAGCT CCTGGAGGTG GGGACGATTG GATCTATGAT TTGGGCATCA 1201 AATATTCGTT TACAATTGAA CTTCGAGATA CGGGCACATA CGGATTCTTG CTGCCGGAGC 1261 GTTACATCAA ACCCACCTGT AGAGAAGCTT TTGCCGCTGT CTCTAAAATA GCTTGGCATG 1321 TCATTAGGAA TGTTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1381 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1441 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCGCTTATAT TCATGCGACG 1501 ACGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt