Construct: ORF TRCN0000466374
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002282.1_s317c1
- Derived from:
- ccsbBroadEn_04005
- DNA Barcode:
- TAAGCGAGCACCTAGCTCAGCAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS34 (65993)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466374
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65993 | MRPS34 | mitochondrial ribosomal pro... | NM_023936.2 | 100% | 100% | |
| 2 | human | 65993 | MRPS34 | mitochondrial ribosomal pro... | NM_001300900.2 | 96.8% | 96.8% | 363_383del |
| 3 | human | 65993 | MRPS34 | mitochondrial ribosomal pro... | XM_017023595.1 | 87.9% | 87.9% | 363_452del |
| 4 | mouse | 79044 | Mrps34 | mitochondrial ribosomal pro... | NM_023260.1 | 83.4% | 89.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 720
- ORF length:
- 654
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcggaagaag gtgcgtccgc ggctgatcgc ggagctggcc cgccgcgtgc 121 gcgccctgcg ggagcaactg aacaggccgc gcgactccca gctctacgcg gtggactacg 181 agaccttgac gcggccgttc tctggacgcc ggctgccggt ccgggcctgg gccgacgtgc 241 gccgcgagag ccgcctcttg cagctgctcg gccgcctccc gctcttcggc ctgggccgcc 301 tggTCACGCG CAAGTCCTGG CTGTGGCAGC ACGACGAGCC GTGCTACTGG CGCCTCACGC 361 GGGTGCGGCC CGACTACACG GCGCAGAACT TGGACCACGG GAAGGCCTGG GGCATCCTGA 421 CCTTCAAAGG GAAGACTGAG AGCGAGGCGC GGGAGATCGA ACACGTCATG TACCATGACT 481 GGCGGCTGGT GCCCAAGCAC GAGGAGGAGG CCTTCACCGC GTTCACGCCG GCGCCGGAAG 541 ACAGCCTGGC CTCCGTGCCG TACCCGCCTC TCCTCCGGGC CATGATTATC GCAGAACGAC 601 AGAAAAATGG AGACACAAGC ACCGAGGAGC CCATGCTGAA TGTGCAGAGG ATACGCATGG 661 AACCCTGGGA TTACCCTGCA AAACAGGAAG ACAAAGGAAG GGCCAAGGGC ACCCCCGTCT 721 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 781 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 841 TTTATATATC TTGTGGAAAG GACGATAAGC GAGCACCTAG CTCAGCAGCA CGCGTTAAGT 901 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt