Construct: ORF TRCN0000466382
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015486.1_s317c1
- Derived from:
- ccsbBroadEn_13749
- DNA Barcode:
- ACTACCCGATGGAAACATAATCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF75A (7627)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466382
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7627 | ZNF75A | zinc finger protein 75a | NM_001324085.1 | 64.1% | 58.1% | (many diffs) |
| 2 | human | 7627 | ZNF75A | zinc finger protein 75a | XM_017023645.2 | 59.7% | 54.2% | (many diffs) |
| 3 | human | 7627 | ZNF75A | zinc finger protein 75a | XM_017023643.2 | 54.3% | 49.4% | (many diffs) |
| 4 | human | 7627 | ZNF75A | zinc finger protein 75a | XM_017023644.2 | 54.3% | 49.4% | (many diffs) |
| 5 | human | 7627 | ZNF75A | zinc finger protein 75a | XM_011522646.3 | 29.9% | 27.4% | (many diffs) |
| 6 | human | 7627 | ZNF75A | zinc finger protein 75a | NM_001302109.1 | 27.8% | 25.5% | (many diffs) |
| 7 | human | 7627 | ZNF75A | zinc finger protein 75a | NM_001324041.1 | 27.8% | 25.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 525
- ORF length:
- 459
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gatggtagat ctgaaagtgg ctgcgtactt ggaccctcag atcagggctt 121 tgtgggagac caaggggcct gcaagagaga gctccggtca gagtaaaaaa tctcctcaaa 181 tggactgtct cgatcctaag agctcttgct ggcacttccg gaatttcacc tatgatgaag 241 caggtggacc ccgtgaggct gtcagcaaac ttcaagaatt atgtcatcta tggctgaagc 301 cagagatcca ctcaaaagag cagatactgg aactgctggt gctggagcag ttcctgacta 361 ttctgcccag ggagacacag acccagatgc agaagcacca tccacagagc attgaggagg 421 ctgtggctct ggtagaacac ttGCAGAGGG AATCTGGTCA AACATGGAAT GGGGTGAGAA 481 GAAAGATTCC TGACATGTAC AGTGAAATAG GATGCAGGTG GATATACCCA ACTTTCTTGT 541 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 601 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 661 GAAAGGACGA ACTACCCGAT GGAAACATAA TCTCACGCGT TAAGTCgaca atcaacctct 721 ggattacaaa atttgtgaaa gatt