Construct: ORF TRCN0000466384
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002964.1_s317c1
- Derived from:
- ccsbBroadEn_06639
- DNA Barcode:
- ATCACAGACGATGAGCTCGGCGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NFYB (4801)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466384
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4801 | NFYB | nuclear transcription facto... | NM_006166.4 | 99.8% | 99.5% | 12T>G |
| 2 | human | 4801 | NFYB | nuclear transcription facto... | XM_024448984.1 | 99.8% | 99.5% | 12T>G |
| 3 | human | 4801 | NFYB | nuclear transcription facto... | XM_024448985.1 | 99.8% | 99.5% | 12T>G |
| 4 | human | 4801 | NFYB | nuclear transcription facto... | XM_005268908.4 | 99% | 98% | 4_5insC;6_9delCCTG;15T>G |
| 5 | human | 4801 | NFYB | nuclear transcription facto... | XM_005268909.4 | 99% | 98% | 4_5insC;6_9delCCTG;15T>G |
| 6 | human | 4801 | NFYB | nuclear transcription facto... | XM_005268910.4 | 99% | 98% | 4_5insC;6_9delCCTG;15T>G |
| 7 | mouse | 18045 | Nfyb | nuclear transcription facto... | NM_010914.2 | 88.7% | 98.5% | (many diffs) |
| 8 | mouse | 18045 | Nfyb | nuclear transcription facto... | XM_011243379.2 | 88.7% | 98.5% | (many diffs) |
| 9 | mouse | 18045 | Nfyb | nuclear transcription facto... | XM_011243380.2 | 55.5% | 61.8% | (many diffs) |
| 10 | mouse | 18045 | Nfyb | nuclear transcription facto... | XM_011243381.2 | 55.5% | 61.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 687
- ORF length:
- 621
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac aatggagggt gacagttcta caacagatgc ttctcaacta ggaatctctg 121 cagactatat tggaggaagt cattatgtta tacagcctca tgatgatact gaggacagca 181 tgaatgatca tgaagacaca aatggttcaa aagaaagttt cagagaaCAA GATATATATC 241 TTCCAATAGC AAACGTGGCT AGGATAATGA AAAATGCCAT ACCTCAAACG GGAAAGATTG 301 CAAAAGATGC CAAAGAATGT GTTCAAGAAT GTGTAAGTGA GTTCATCAGT TTTATAACAT 361 CTGAAGCAAG TGAAAGGTGC CATCAAGAGA AACGGAAAAC AATCAATGGA GAAGATATTC 421 TCTTTGCTAT GTCTACTTTA GGCTTTGACA GTTATGTGGA ACCTCTGAAA TTATACCTTC 481 AGAAATTCAG AGAGGCTATG AAAGGAGAAA AGGGAATTGG TGGAGCAGTC ACAGCTACAG 541 ATGGACTAAG TGAAGAGCTT ACAGAGGAGG CATTTACTAA CCAGTTACCA GCTGGCTTAA 601 TAACCACAGA CGGTCAACAA CAAAATGTTA TGGTTTACAC AACATCATAT CAACAGATTT 661 CTGGTGTTCA GCAAATTCAG TTTTCATGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 721 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 781 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATCACAGA 841 CGATGAGCTC GGCGGGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 901 aagatt