Transcript: Mouse XM_011243380.2

PREDICTED: Mus musculus nuclear transcription factor-Y beta (Nfyb), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Nfyb (18045)
Length:
2423
CDS:
311..709

Additional Resources:

NCBI RefSeq record:
XM_011243380.2
NBCI Gene record:
Nfyb (18045)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011243380.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000285137 AGTTACCAGCTGGCTTAATAA pLKO_005 603 CDS 100% 15.000 21.000 N NFYB n/a
2 TRCN0000349712 AGTTACCAGCTGGCTTAATAA pLKO_005 603 CDS 100% 15.000 21.000 N Nfyb n/a
3 TRCN0000312834 TACATCGGAGGAAGTCATTAT pLKO_005 277 5UTR 100% 13.200 18.480 N Nfyb n/a
4 TRCN0000312787 CAACGTCTTACCAACAGATTT pLKO_005 660 CDS 100% 13.200 9.240 N Nfyb n/a
5 TRCN0000274068 TTGACTAATTGAGGTGTTAAT pLKO_005 839 3UTR 100% 13.200 9.240 N NFYB n/a
6 TRCN0000312855 TTGACTAATTGAGGTGTTAAT pLKO_005 839 3UTR 100% 13.200 9.240 N Nfyb n/a
7 TRCN0000084491 CACAACGTCTTACCAACAGAT pLKO.1 658 CDS 100% 4.950 3.465 N Nfyb n/a
8 TRCN0000084490 CCAAAGAATGTGTTCAGGAAT pLKO.1 330 CDS 100% 4.950 3.465 N Nfyb n/a
9 TRCN0000311789 CCAAAGAATGTGTTCAGGAAT pLKO_005 330 CDS 100% 4.950 3.465 N Nfyb n/a
10 TRCN0000084492 GCTAGGGATTTCTGCAGACTA pLKO.1 258 5UTR 100% 4.950 3.465 N Nfyb n/a
11 TRCN0000084489 GCTGGCTTAATAACTGCAGAT pLKO.1 611 CDS 100% 4.050 2.835 N Nfyb n/a
12 TRCN0000084488 CGAGAGTTTGTTGCTCTGTAT pLKO.1 944 3UTR 100% 4.950 2.970 N Nfyb n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011243380.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06639 pDONR223 100% 55.5% 61.8% None (many diffs) n/a
2 ccsbBroad304_06639 pLX_304 0% 55.5% 61.8% V5 (many diffs) n/a
3 TRCN0000466384 ATCACAGACGATGAGCTCGGCGGG pLX_317 73.3% 55.5% 61.8% V5 (many diffs) n/a
4 ccsbBroadEn_15506 pDONR223 0% 55.5% 61.8% None (many diffs) n/a
5 ccsbBroad304_15506 pLX_304 0% 55.5% 61.8% V5 (many diffs) n/a
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