Construct: ORF TRCN0000466392

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014617.3_s317c1
Derived from:
ccsbBroadEn_15150
DNA Barcode:
CCCCACCTGTCGTCTGTACTGGGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FASTKD1 (79675)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466392
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79675 FASTKD1 FAST kinase domains 1 XM_017004911.2 99.9% 100% 1161A>G
2 human 79675 FASTKD1 FAST kinase domains 1 NM_001322048.1 81.8% 81.9% 1_447del;1608A>G
3 human 79675 FASTKD1 FAST kinase domains 1 XM_006712750.4 81.8% 81.9% 1_447del;1608A>G
4 human 79675 FASTKD1 FAST kinase domains 1 NM_001322046.1 79.6% 79.6% 1_516del;1677A>G
5 human 79675 FASTKD1 FAST kinase domains 1 NM_024622.6 79.6% 79.6% 1_516del;1677A>G
6 human 79675 FASTKD1 FAST kinase domains 1 XM_006712751.4 77.7% 77.4% (many diffs)
7 human 79675 FASTKD1 FAST kinase domains 1 NM_001322049.1 76.6% 76.5% 1_447del;1608A>G;1876_1877ins129
8 human 79675 FASTKD1 FAST kinase domains 1 NM_001281476.2 74.5% 74.4% 1_516del;1677A>G;1945_1946ins129
9 human 79675 FASTKD1 FAST kinase domains 1 XM_017004912.2 71.3% 70.9% (many diffs)
10 human 79675 FASTKD1 FAST kinase domains 1 NR_104020.2 44.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2091
ORF length:
2025
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg aaaaatagct gatattgttc ataggaactt ggaaaccaca caggacttaa
121gttccttgtc tgtcttgatg gtcaacatat cttctttaat atcacgacat tttcaacaac
181aactggtgaa caaaacagaa cttctttttg acaccataga ttcttctgag gtcaacgttg
241caaaaagcat agcaaagttt cttcgaaatg ttagatatcg ttatcaacca ctattagaaa
301gatgtaataa cgtattttta agtaatgtgg accaccttga tttggattcc atcagtaaaa
361tacttagtgt atacaaattt ctacaattta atagttttga atttattata atggctaaaa
421agaagctaac tgaaatgatt cctctgtgta atcatcctgc tagctttgta aaattgtttg
481tagcattggg acccattgca ggacctgaag aaaagaaaca acttaaatca actatgttat
541tgatgtcaga ggacctaact ggcgagcaag ccctggcagt gttgggagca atgggagata
601tggaaagcag aaactcatgt ctgattaaaa gagttacttc agttctgcat aaacatttgg
661atggctataa accattagag ttgttgaaga taactcaaga attaactttt ctgcatttcc
721aaaggaagga gttttttgcg aaacttagag aattactgct tagttatttg aaaaatagtt
781tcataccaac tgaggtgtct gttctggtcc gtgctatttc cctgctccct tctcctcact
841tggacgaagt ggggatatcc cgaattgaag ccgttttacc acagtgtgac ctaaataacc
901tgagtagttt tgccacatct gttttaagat ggattcagca tgatcacatg tatttggata
961atatgactgc gaaacaactg aaactacttc aaaaattaga tcactatggt cgtcagagac
1021tacaacacag caacagtttg gatctgttac ggaaggaact taaatctctc aaaggaaaca
1081cgtttcctga gtcacttctt gaagaaatga ttgctacttt acagcatttc atggatgata
1141ttaattacat aaatgttggg gagattgcat cttttatttc tagtactgat tacctcagta
1201ctttgctact agataggata gcctcggtgg ctgttcagca gattgaaaag atccatcctt
1261ttacaatccc tgctattatt cgtccattca gcgtattgaa ctatgatcca cctcaaaggg
1321atgaattttt gggaacttgc gtgcaacatc ttaattctta cttaggtata ttggatcctt
1381ttatattagt gtttcttggt ttctctttgg ccacacttga atattttcca gaagatctgc
1441taaaggcaat ttttaacatc aaattcttag ctagattgga ttctcaactt gaaattttat
1501ctccatctcg aagtgcaaga gtccagtttc atcttatgga gttaaataga tcagtctgct
1561tggaatgccc tgagtttcag attccatggt ttcatgaccg cttctgtcaa caatataata
1621aaggtattgg tggcatggat ggaacacaac agcagatttt taaaatgtta gcagaggtac
1681taggaggaat caattgtgta aaagcctcgg ttcttacgcc ttattaccac aaagtagatt
1741ttgagtgtat cttggataaa agaaaaaaac cTCTTCCGTA TGGAAGCCAT AATATAGCAT
1801TGGGACAACT ACCAGAAATG CCCTGGGAAT CAAATATCGA AATAGTTGGA TCAAGGCTGC
1861CACCAGGGGC TGAAAGGATT GCTTTGGAAT TTTTGGATTC AAAAGCACTT TGTAGAAATA
1921TCCCTCACAT GAAAGGAAAA TCTGCTATGA AAAAACGACA TTTGGAAATT CTGGGGTATC
1981GTGTAATTCA GATTTCCCAG TTTGAATGGA ACTCTATGGC ACTGTCAACA AAGGATGCTC
2041GGATGGACTA CCTGAGAGAA TGTATATTTG GAGAAGTCAA GTCATGTTTG TACCCAACTT
2101TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
2161CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
2221CTTGTGGAAA GGACGACCCC ACCTGTCGTC TGTACTGGGC ACGCGTTAAG TCgacaatca
2281acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)

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