Construct: ORF TRCN0000466395
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004114.1_s317c1
- Derived from:
- ccsbBroadEn_06883
- DNA Barcode:
- ACACTTCCGAAAATTGCCGAATAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL18A (6142)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466395
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6142 | RPL18A | ribosomal protein L18a | NM_000980.4 | 99.8% | 100% | 306C>A |
| 2 | mouse | 76808 | Rpl18a | ribosomal protein L18A | NM_029751.4 | 88.8% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 594
- ORF length:
- 528
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggcctcgggc acgctacgag agtacaaggt agtgggtcgc tgcctgccca 121 cccccaaatg ccacacgccg cccctctacc gcatgcgaat ctttgcgcct aatcatgtcg 181 tcgccaagtc ccgcttctgg tactttgtat ctcagttaaa gaagatgaag aagtcttcag 241 gggagattgt ctactgtggg caggtgtttg agaagtcccc ccTGCGGGTG AAGAACTTCG 301 GGATCTGGCT GCGCTATGAC TCCCGGAGCG GCACCCACAA CATGTACCGG GAATACCGGG 361 ACCTGACCAC AGCAGGCGCT GTCACCCAGT GCTACCGAGA CATGGGTGCC CGGCACCGCG 421 CCCGAGCCCA CTCCATTCAG ATCATGAAGG TGGAGGAGAT CGCGGCCAGC AAGTGCCGCC 481 GGCCGGCTGT CAAGCAGTTC CACGACTCCA AGATCAAGTT CCCGCTGCCC CACCGGGTCC 541 TGCGCCGTCA GCACAAGCCA CGCTTCACCA CCAAGAGGCC CAACACCTTC TTCTACCCAA 601 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 661 ATTCTACGTA GTAATGAACT AGCCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 721 TATCTTGTGG AAAGGACGAA CACTTCCGAA AATTGCCGAA TAGACGCGTT AAGTCgacaa 781 tcaacctctg gattacaaaa tttgtgaaag att