Construct: ORF TRCN0000466418
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003789.1_s317c1
- Derived from:
- ccsbBroadEn_13222
- DNA Barcode:
- ACAACTCCCGTATCTCCTGCTAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ETFRF1 (144363)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466418
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144363 | ETFRF1 | electron transfer flavoprot... | NM_001001660.3 | 97.7% | 97.7% | 1_6delATGAAA |
| 2 | human | 144363 | ETFRF1 | electron transfer flavoprot... | XM_005253319.4 | 97.7% | 97.7% | 1_6delATGAAA |
| 3 | human | 144363 | ETFRF1 | electron transfer flavoprot... | XM_005253320.4 | 97.7% | 97.7% | 1_6delATGAAA |
| 4 | human | 144363 | ETFRF1 | electron transfer flavoprot... | XM_017018850.2 | 97.7% | 97.7% | 1_6delATGAAA |
| 5 | mouse | 67636 | Etfrf1 | electron transfer flavoprot... | NM_001163628.1 | 87.2% | 88.6% | (many diffs) |
| 6 | mouse | 67636 | Etfrf1 | electron transfer flavoprot... | NM_133688.3 | 87.2% | 88.6% | (many diffs) |
| 7 | mouse | 67636 | Etfrf1 | electron transfer flavoprot... | XM_006506982.2 | 87.2% | 88.6% | (many diffs) |
| 8 | mouse | 67636 | Etfrf1 | electron transfer flavoprot... | XM_006506983.3 | 87.2% | 88.6% | (many diffs) |
| 9 | mouse | 67636 | Etfrf1 | electron transfer flavoprot... | XM_006506984.2 | 87.2% | 88.6% | (many diffs) |
| 10 | mouse | 67636 | Etfrf1 | electron transfer flavoprot... | XM_017321721.1 | 87.2% | 88.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 354
- ORF length:
- 264
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCCAATTC TTTAAGAGGA GAAGTACTAA 121 AACTTTATAA AAATCTGCTG TATCTTGGAC GAGACTATCC AAAAGGAGCA GACTATTTTA 181 AAAAGCGTTT GAAGAACATT TTCCTTAAAA ACAAAGATGT GAAGAATCCA GAGAAGATCA 241 AAGAACTTAT TGCACAGGGC GAATTTGTAA TGAAAGAGCT AGAAGCTTTG TACTTCCTTA 301 GGAAATACAG AGCTATGAAA CAACGCTATT ATTCAGATAC CAACAAAACT AATTGCCCAA 361 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 421 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 481 TATCTTGTGG AAAGGACGAA CAACTCCCGT ATCTCCTGCT AAGACGCGTT AAGTCgacaa 541 tcaacctctg gattacaaaa tttgtgaaag att