Construct: ORF TRCN0000466419
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005169.1_s317c1
- Derived from:
- ccsbBroadEn_12694
- DNA Barcode:
- TAAAGTGCATACCCCAACCCCCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAM33 (80332)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466419
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | XM_017028083.1 | 21.3% | 21.2% | 255_1188delinsG |
| 2 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | XM_017028082.1 | 19.4% | 19.3% | 255_1305delinsG |
| 3 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | XM_006723644.2 | 17.3% | 17.1% | 255_1467delinsG |
| 4 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | XM_017028081.2 | 15.4% | 15.3% | 255_1641delinsG |
| 5 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | XM_011529371.2 | 15.1% | 15% | 255_1680delinsG |
| 6 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | XM_017028080.2 | 15.1% | 15% | 255_1680delinsG |
| 7 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | XM_011529370.1 | 13.3% | 13.2% | 255_1905delinsG |
| 8 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | NM_153202.3 | 10.7% | 10.6% | 255_2361delinsG |
| 9 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | XM_011529367.1 | 10.4% | 10.4% | 256_2436del |
| 10 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | NM_001282447.2 | 10.4% | 10.3% | 255_2436delinsG |
| 11 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | NM_025220.4 | 10.4% | 10.3% | 255_2439delinsG |
| 12 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | XM_011529366.1 | 10.3% | 10.3% | 256_2475del |
| 13 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | XM_006723640.1 | 10.2% | 10.2% | 255_2469delinsG |
| 14 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | XM_005260843.1 | 10.2% | 10.1% | 255_2478delinsG |
| 15 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | XR_937152.1 | 10.2% | 1_104del;359_2482delinsG | |
| 16 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | XR_937151.1 | 10.2% | 1_104del;359_2483delinsG | |
| 17 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | XR_001754405.1 | 7.1% | 1_104del;359_3540delinsG | |
| 18 | human | 80332 | ADAM33 | ADAM metallopeptidase domai... | XR_002958534.1 | 6.9% | 1_104del;359_3649delinsG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 345
- ORF length:
- 255
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGGCTGGAG GCCCCGGAGA GCTCGGGGGA 121 CCCCGTTGCT GCTGCTGCTA CTACTGCTGC TGCTCTGGCC AGTGCCAGGC GCCGGGGTGC 181 TTCAAGGACA TATCCCTGGG CAGCCAGTCA CCCCGCACTG GGTCCTGGAT GGACAACCCT 241 GGCGCACCGT CAGCCTGGAG GAGCCGGTCT CGAAGCCAGA CATGGGGCTG GTGGCCCTGG 301 AGGCTGAAGG CCAGGAGCTC CTGCTTGAGC TGGAGAAGAA CCAGTGCCCA ACTTTCTTGT 361 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 421 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 481 GAAAGGACGA TAAAGTGCAT ACCCCAACCC CCTGACGCGT TAAGTCgaca atcaacctct 541 ggattacaaa atttgtgaaa gatt