Construct: ORF TRCN0000466422
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002026.1_s317c1
- Derived from:
- ccsbBroadEn_13418
- DNA Barcode:
- CACAAGGAGCGTGCCTGTATGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RASGEF1A (221002)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466422
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221002 | RASGEF1A | RasGEF domain family member 1A | XM_005271809.3 | 93.5% | 93.4% | 0_1ins81;501C>A;1153A>G |
| 2 | human | 221002 | RASGEF1A | RasGEF domain family member 1A | XM_011539500.2 | 93.5% | 93.4% | 0_1ins81;501C>A;1153A>G |
| 3 | human | 221002 | RASGEF1A | RasGEF domain family member 1A | NM_145313.4 | 88.8% | 88.7% | 1_159del;741C>A;1393A>G |
| 4 | human | 221002 | RASGEF1A | RasGEF domain family member 1A | NM_001282862.1 | 87.3% | 87.3% | 1_183del;765C>A;1417A>G |
| 5 | mouse | 70727 | Rasgef1a | RasGEF domain family, membe... | NM_001316749.1 | 79.4% | 84.4% | (many diffs) |
| 6 | mouse | 70727 | Rasgef1a | RasGEF domain family, membe... | XM_017321738.1 | 79.4% | 84.4% | (many diffs) |
| 7 | mouse | 70727 | Rasgef1a | RasGEF domain family, membe... | NM_027526.1 | 78.1% | 83% | (many diffs) |
| 8 | mouse | 70727 | Rasgef1a | RasGEF domain family, membe... | XM_017321737.1 | 77.7% | 82.5% | (many diffs) |
| 9 | mouse | 70727 | Rasgef1a | RasGEF domain family, membe... | XM_006506596.3 | 68.1% | 67.9% | (many diffs) |
| 10 | mouse | 70727 | Rasgef1a | RasGEF domain family, membe... | XM_006506597.3 | 68.1% | 67.9% | (many diffs) |
| 11 | mouse | 70727 | Rasgef1a | RasGEF domain family, membe... | XM_006506595.3 | 67.1% | 66.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1350
- ORF length:
- 1284
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcaccttgtt cccacggtgg actattaccc cgataggacg tacatcttca 121 cctttctcct gagctcccgg gtctttatgc cccctcatga cctgctggcc cgcgtggggc 181 agatctgcgt ggagcagaag cagcagctgg aagccgggcc tgaaaaggcc aagctgaagt 241 ctttctcagc caagatcgtg cagctcctga aggagtggac cgaggccttc ccctatgact 301 tccaggatga gaaggccatg gccgagctga aagccatcac acaccgtgtc acccagtgtg 361 atgaggagaa tggcacagtg aagaaggcca ttgcccagat gacacagagc ctgttgctgt 421 ccttggctgc ccggagccag ctccaggaac tgcgagagaa gctccggcca ccggctgtag 481 acaaggggcc catcctcaag accaagccac cagccgccca gaaggacatc ctgggcgtgt 541 gctgcgaccc cctggtgctg gcccagcagc tgactcacat tgagctggac agggtcagca 601 gcatttaccc tgaggacttg atgcagatcg tcagccacat ggactcattg gacaaccaca 661 ggtgccgagg ggacctgacc aagacctaca gcctggaggc ctatgacaac tggttcaact 721 gcctgagcat gctggtggcc actgaggtgt gccgggtggt gaagaagaaa caccggaccc 781 gcatgttgga gttcttcatt gatgtggccc gggagtgctt caacatcggg aacttcaact 841 ccatgatggc catcatctct ggcatgaacc tcagtcctgt ggcaaggctg aagaaaactt 901 ggtccaaggt caagacagcc aagtttgatg tcttGGAGCA TCACATGGAC CCGTCCAGCA 961 ACTTCTGCAA CTACCGTACA GCCCTGCAGG GGGCCACGCA GAGGTCCCAG ATGGCCAACA 1021 GCAGCCGTGA AAAGATCGTC ATCCCTGTGT TCAACCTCTT CGTTAAGGAC ATCTACTTCC 1081 TGCACAAAAT CCATACCAAC CACCTGCCCA ACGGGCACAT TAACTTTAAG AAATTCTGGG 1141 AGATCTCCAG ACAGATCCAT GAGTTCATGA CATGGACACA GGTAGAGTGT CCTTTCGAGA 1201 AGGACAAGAA GATTCAGAGT TACCTGCTCA CGGCGCCCAT CTACAGCGAG GAAGCTCTCT 1261 TCGTCGCCTC CTTTGAAAGT GAGGGTCCCG AGAACCACGT GGAAAAAGAC AGCTGGAAGA 1321 CCCTCAGGAC CACCCTTCTG AACAGAGCCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1381 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1441 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACACAA 1501 GGAGCGTGCC TGTATGTTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1561 tgaaagatt