Construct: ORF TRCN0000466441
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018827.3_s317c1
- Derived from:
- ccsbBroadEn_15335
- DNA Barcode:
- TACTAAGGAGAGATAGGTAGTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AK4P6 (649288)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466441
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 205 | AK4 | adenylate kinase 4 | NM_001330616.2 | 40.3% | 37% | (many diffs) |
| 2 | human | 205 | AK4 | adenylate kinase 4 | XM_017000613.1 | 40.3% | 37% | (many diffs) |
| 3 | human | 205 | AK4 | adenylate kinase 4 | NM_001005353.2 | 40.2% | 35.4% | (many diffs) |
| 4 | human | 205 | AK4 | adenylate kinase 4 | NM_013410.4 | 40.2% | 35.4% | (many diffs) |
| 5 | human | 205 | AK4 | adenylate kinase 4 | NM_203464.2 | 40.2% | 35.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 360
- ORF length:
- 291
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaagtcttG GAATCATTTA CCAAGAAGAT CCTGAGCACA GAAGTTGGTG 121 AGATGGCAAA GCAGTATATA GAGAAAAGTC TTTTGGTTCC AGACCATGTG ATCACATACC 181 TAATGATGTC TGAGTTGGAG AATAGGTGTG GCCAGCACTG GCTCCTCGAT GGTTTTCCTA 241 GGACATTAGG ACAAGCCGAA GCCCTGGACA AAATCTGTGA AGTGGATCTA GTGATCAGTT 301 TGAACATTCC ATTTGAAACA CTTAAAAGAT CGTCTCAGCC GCCGTTGGAT TCACCCTCCT 361 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 421 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 481 TTTATATATC TTGTGGAAAG GACGATACTA AGGAGAGATA GGTAGTATCA CGCGTTAAGT 541 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt