Construct: ORF TRCN0000466452
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003565.1_s317c1
- Derived from:
- ccsbBroadEn_10212
- DNA Barcode:
- CGTTCTAAGCAATTTTCCATACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRHL3 (57822)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466452
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57822 | GRHL3 | grainyhead like transcripti... | NM_001195010.2 | 99.8% | 99.8% | 1_3delATG |
| 2 | human | 57822 | GRHL3 | grainyhead like transcripti... | XM_011541869.1 | 99.8% | 99.8% | 1_3delATG |
| 3 | human | 57822 | GRHL3 | grainyhead like transcripti... | NM_198173.3 | 92.1% | 92.1% | 1_141del |
| 4 | human | 57822 | GRHL3 | grainyhead like transcripti... | XM_011541870.2 | 91.7% | 91.7% | 0_1ins138 |
| 5 | human | 57822 | GRHL3 | grainyhead like transcripti... | NM_021180.3 | 91.4% | 91.4% | 1_156del |
| 6 | human | 57822 | GRHL3 | grainyhead like transcripti... | NM_198174.3 | 85% | 83.7% | (many diffs) |
| 7 | mouse | 230824 | Grhl3 | grainyhead-like 3 (Drosophila) | XM_006538760.3 | 80.5% | 84.3% | (many diffs) |
| 8 | mouse | 230824 | Grhl3 | grainyhead-like 3 (Drosophila) | NM_001013756.1 | 80.3% | 84.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1731
- ORF length:
- 1665
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag agtcaatgga gatgatgaca gtgttgcggc cttgagcttc ctctatgatt 121 actacatggg tcccaaggag aagcggatat tgtcctccag cactgggggc aggaatgacc 181 aaggaaagag gtactaccat ggcatggaat atgagacgga cctcactccc cttgaaagcc 241 ccacacacct catgaaattc ctgacagaga acgtgtctgg aaccccagag tacccagatt 301 tgctcaagaa gaataacctg atgagcttgg agggggcctt gcccacccct ggcaaggcag 361 ctcccctccc tgcaggcccc agcaagctgg aggccggctc tgtggacagc tacctgttac 421 ccaccactga tatgtatgat aatggctccc tcaactcctt gtttgagagc attcatgggg 481 tgccgcccac acagcgctgg cagccagaca gcaccttcaa agatgaccca caggagtcga 541 tgctcttccc agatatcctg aaaacctccc cggaaccccc atgtccagag gactacccca 601 gcctcaaaag tgactttgaa tacaccctgg gctcccccaa agccatccac atcaagtcag 661 gcgagtcacc catggcctac ctcaacaaag gccagttcta ccccgtcacc ctgcggaccc 721 cagcaggtgg caaaggcctt gccttgtcct ccaacaaagt caagagtgtg gtgatggttg 781 tcttcgacaa tgagaaggtc ccagtagagc agctgcgctt ctggaagcac tggcattccc 841 ggcaacccac tgccaagcag cgggtcattg acgtggctga ctgcaaagaa aacttcaaca 901 ctgtggagca cattgaggag gtggcctata atgcactgtc ctttgtgtgg aacgtgaatg 961 aagaggccaa ggtgttcatc ggcgtaaact gtctgagcac agacttttcc tcacaaaagg 1021 gggtgaaggg tgtccccctg aacctgcaga ttgacaccta tgactgtggc ttgggcactg 1081 agcgcctggt acaccgtgct gtctgccaga tcaagatctt ctgtgacaag ggagctgaga 1141 ggaagatgcg cgatgacgag cggaagcagt tccggaggaa ggtcaagtgc cctgactcca 1201 gcaacagtgg cgtcaagggc tgcctgctgt cgggcttcag gggcaatgag acgacctacc 1261 ttcggccaga gactgacctg gagacgccac ccgtgctgtt catccccaat gtgcacttct 1321 ccagcctgca gcgctctgga ggggcagccc cctcggcagg acccagcagc tccaacaggc 1381 tgcctctgaa gcgtacctgc tcgcccttca ctgaggagtt tgagcctctg ccctccaagc 1441 aggccaagga aggcgacctT CAGAGAGTTC TGCTGTATGT GCGGAGGGAG ACTGAGGAGG 1501 TGTTTGACGC GCTCATGTTG AAGACCCCAG ACCTGAAGGG GCTGAGGAAT GCGATCTCTG 1561 AGAAGTATGG GTTCCCTGAA GAGAACATTT ACAAAGTCTA CAAGAAATGC AAGCGAGGAA 1621 TCTTAGTCAA CATGGACAAC AACATCATTC AGCATTACAG CAACCACGTC GCCTTCCTGC 1681 TGGACATGGG GGAGCTGGAC GGCAAAATTC AGATCATCCT TAAGGAGCTG TACCCAACTT 1741 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1801 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1861 CTTGTGGAAA GGACGACGTT CTAAGCAATT TTCCATACAT ACGCGTTAAG TCgacaatca 1921 acctctggat tacaaaattt gtgaaagatt