Construct: ORF TRCN0000466453
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016552.1_s317c1
- Derived from:
- ccsbBroadEn_06740
- DNA Barcode:
- AAAATTTTCTGTCCTGACGAACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLP1 (5354)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466453
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5354 | PLP1 | proteolipid protein 1 | NM_199478.3 | 99.8% | 100% | 504T>C |
| 2 | human | 5354 | PLP1 | proteolipid protein 1 | NM_000533.5 | 87.2% | 87.3% | 348_452del;609T>C |
| 3 | human | 5354 | PLP1 | proteolipid protein 1 | NM_001128834.2 | 87.2% | 87.3% | 348_452del;609T>C |
| 4 | human | 5354 | PLP1 | proteolipid protein 1 | NM_001305004.1 | 66.9% | 67.1% | (many diffs) |
| 5 | mouse | 18823 | Plp1 | proteolipid protein (myelin) 1 | NM_001290561.1 | 96.4% | 100% | (many diffs) |
| 6 | mouse | 18823 | Plp1 | proteolipid protein (myelin) 1 | XM_017318432.1 | 96.4% | 100% | (many diffs) |
| 7 | mouse | 18823 | Plp1 | proteolipid protein (myelin) 1 | NM_011123.3 | 84.2% | 87.3% | (many diffs) |
| 8 | mouse | 18823 | Plp1 | proteolipid protein (myelin) 1 | XM_017318430.1 | 84.2% | 87.3% | (many diffs) |
| 9 | mouse | 18823 | Plp1 | proteolipid protein (myelin) 1 | XM_017318431.1 | 84.2% | 87.3% | (many diffs) |
| 10 | mouse | 18823 | Plp1 | proteolipid protein (myelin) 1 | NM_001290562.1 | 71.9% | 60.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 792
- ORF length:
- 726
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cttgttagag tgctgtgcaa gatgtctggt aggggccccc tttgcttccc 121 tggtggccac tggattgtgt ttctttgggg tggcactgtt ctgtggctgt ggacatgaag 181 ccctcactgg cacagaaaag ctaattgaga cctatttctc caaaaactac caagactatg 241 agtatctcat caatgtgatc catgccttcc agtatgtcat ctatggaact gcctctttct 301 tcttccttta tggggccctc ctgctggctg agggcttcta caccaccggc gcagtcaggc 361 agatctttGG CGACTACAAG ACCACCATCT GCGGCAAGGG CCTGAGCGCA ACGTTTGTGG 421 GCATCACCTA TGCCCTGACC GTTGTGTGGC TCCTGGTGTT TGCCTGCTCT GCTGTGCCTG 481 TGTACATTTA CTTCAACACC TGGACCACCT GCCAGTCTAT TGCCTTCCCC AGCAAGACCT 541 CTGCCAGTAT AGGCAGTCTC TGTGCTGACG CCAGAATGTA TGGTGTTCTC CCATGGAATG 601 CTTTCCCTGG CAAGGTTTGT GGCTCCAACC TTCTGTCCAT CTGCAAAACA GCTGAGTTCC 661 AAATGACCTT CCACCTGTTT ATTGCTGCAT TTGTGGGGGC TGCAGCTACA CTGGTTTCCC 721 TGCTCACCTT CATGATTGCT GCCACTTACA ACTTTGCCGT CCTTAAACTC ATGGGCCGAG 781 GCACCAAGTT CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 841 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 901 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAA ATTTTCTGTC CTGACGAACG 961 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t