Transcript: Mouse XM_017318432.1

PREDICTED: Mus musculus proteolipid protein (myelin) 1 (Plp1), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Plp1 (18823)
Length:
3305
CDS:
352..1080

Additional Resources:

NCBI RefSeq record:
XM_017318432.1
NBCI Gene record:
Plp1 (18823)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017318432.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000090336 CACCTGTTTATTGCTGCGTTT pLKO.1 958 CDS 100% 4.050 5.670 N Plp1 n/a
2 TRCN0000090334 GCTTTCCAGTATGTCATCTAT pLKO.1 550 CDS 100% 5.625 3.938 N Plp1 n/a
3 TRCN0000084077 GCAGATCTTTGGCGACTACAA pLKO.1 645 CDS 100% 4.950 3.465 N PLP1 n/a
4 TRCN0000084076 CCTTCATGATTGCTGCCACTT pLKO.1 1013 CDS 100% 4.050 2.835 N PLP1 n/a
5 TRCN0000090333 CCCATGAAATTATTGGGAATT pLKO.1 2292 3UTR 100% 0.000 0.000 N Plp1 n/a
6 TRCN0000424960 GAGAGTCTTGCAGTGATTAAG pLKO_005 1206 3UTR 100% 13.200 9.240 N PLP1 n/a
7 TRCN0000166364 CACACACACACACACACACAA pLKO.1 3125 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017318432.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06740 pDONR223 100% 96.4% 100% None (many diffs) n/a
2 ccsbBroad304_06740 pLX_304 0% 96.4% 100% V5 (many diffs) n/a
3 TRCN0000466453 AAAATTTTCTGTCCTGACGAACGA pLX_317 58.1% 96.4% 100% V5 (many diffs) n/a
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