Construct: ORF TRCN0000466460
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001304.1_s317c1
- Derived from:
- ccsbBroadEn_09679
- DNA Barcode:
- CAATTACAGAATAACGTAGCTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL23 (151230)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466460
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100526832 | PHOSPHO2-KLHL23 | PHOSPHO2-KLHL23 readthrough | NM_001199290.3 | 99.9% | 100% | 1416C>T |
| 2 | human | 151230 | KLHL23 | kelch like family member 23 | NM_144711.6 | 99.9% | 100% | 1416C>T |
| 3 | mouse | 277396 | Klhl23 | kelch-like 23 | NM_177784.4 | 90.1% | 97.1% | (many diffs) |
| 4 | mouse | 277396 | Klhl23 | kelch-like 23 | XM_006499677.1 | 90.1% | 97.1% | (many diffs) |
| 5 | mouse | 277396 | Klhl23 | kelch-like 23 | XM_006499678.3 | 90.1% | 97.1% | (many diffs) |
| 6 | mouse | 277396 | Klhl23 | kelch-like 23 | XR_374475.1 | 39.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1740
- ORF length:
- 1674
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tctaaaagga caagaagatt atatttatct tttcaaggat tcaacacatc 121 cagtggattt tctggatgca ttcagaacat tttacttgga tggattattt actgatatta 181 ctcttcagtg tccttcaggc ataattttcc attgtcaccg agccgtttta gctgcttgca 241 gcaattattt taaggcaatg ttcacagctg acatgaaaga aaaatttaaa aataaaataa 301 aactctctgg catccaccat gatattctgg aaggccttgt aaattatgca tacacttccc 361 aaattgaaat aactaaaaga aatgttcaaa gcctgcttga ggcagcggat ctgctacagt 421 tcctttcagt aaagaaggct tgtgagcggt ttttggtaag gcacttggat attgataatt 481 gtattggaat gcactccttt gcagaatttc atgtgtgtcc agaactagag aaggaatctc 541 gaagaattct atgttcaaag tttaaggaag tgtggcaaca agaagaattt ctggaaatca 601 gccttgaaaa gtttctcttt atcttgtcca gaaagaatct cagtgtttgg aaagaagaag 661 ctatcataga gccagttatt aagtggactg ctcatgatgt agaaaatcga attgaatgcc 721 tctataatct actgagctat atcaacattg atatagatcc agtgtactta aaaacagcct 781 taggccttca aagaagctgc ctgctcaccg aaaataagat ccgctcccta atatacaatg 841 ccttgaatcc catgcataaa gagatttccc agaggtccac agccacaatg tatataattg 901 gaggctatta ctggcatcct ttatcagagg ttcacatatg ggatcctttg acaaatgttt 961 ggattcaggg agcagaaata ccagattata ccagggagag ctatggtgtt acatgtttag 1021 gacccaacat ttatgtaact gggggctaca ggacggataa catagaagct cttgacacag 1081 tgtggatcta taacagtgaa agtgatgaat ggacagaagg tttgccaatg ctcaatgcca 1141 ggtattacca ctgtgcagtc accttgggtg gctgtgtcta tgctttaggt ggttacagaa 1201 aaggggctcc agcagaagag gctgagttct atgatccttt aaaagagaaa tggattccta 1261 ttgcaaacat gattaaaggt gtgggaaatg ctactgcctg tgtcttacat gatgttatct 1321 acgtcattgg tggccactgt ggctacagag gaagctgcac ctatgacaaa gttcagagct 1381 acaattccga tatcaacgaa tggagcctca TCACCTCCAG TCCACATCCA GAATATGGAT 1441 TGTGCTCAGT TCCGTTTGAA AATAAGCTCT ATCTAGTCGG TGGACAAACT ACAATCACAG 1501 AATGCTATGA CCCTGAACAA AATGAATGGA GAGAGATAGC TCCCATGATG GAAAGGAGGA 1561 TGGAGTGCGG TGCCGTCATC ATGAATGGAT GTATTTATGT CACTGGAGGA TACTCCTACT 1621 CAAAGGGAAC GTATCTTCAG AGCATTGAGA AATATGATCC AGATCTTAAT AAGTGGGAAA 1681 TAGTGGGTAA TCTTCCCAGT GCCATGCGGT CTCATGGGTG TGTTTGTGTG TATAATGTCT 1741 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1801 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1861 TTTATATATC TTGTGGAAAG GACGACAATT ACAGAATAAC GTAGCTAACA CGCGTTAAGT 1921 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt