Construct: ORF TRCN0000466476

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014803.1_s317c1
Derived from:
ccsbBroadEn_14130
DNA Barcode:
AGGGGTTTGCTCTTCAAAACCCAT
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PNPLA8 (50640)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466476
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 50640 PNPLA8 patatin like phospholipase ... NM_001256007.3 99.9% 99.4% 2332delT
2 human 50640 PNPLA8 patatin like phospholipase ... NM_001256008.3 99.9% 99.4% 2332delT
3 human 50640 PNPLA8 patatin like phospholipase ... NM_015723.5 99.9% 99.4% 2332delT
4 human 50640 PNPLA8 patatin like phospholipase ... XM_011516274.3 94.7% 94.3% (many diffs)
5 human 50640 PNPLA8 patatin like phospholipase ... NM_001256009.3 92% 91.5% 1692_1693ins186;2146delT
6 human 50640 PNPLA8 patatin like phospholipase ... XM_005250396.5 92% 91.5% 1692_1693ins186;2146delT
7 human 50640 PNPLA8 patatin like phospholipase ... XM_011516275.3 87.1% 86.6% (many diffs)
8 human 50640 PNPLA8 patatin like phospholipase ... NM_001256010.3 87.1% 86.7% 0_1ins300;2032delT
9 human 50640 PNPLA8 patatin like phospholipase ... NM_001256011.3 87.1% 86.7% 0_1ins300;2032delT
10 human 50640 PNPLA8 patatin like phospholipase ... XM_017012261.2 79.2% 78.7% 0_1ins300;1392_1393ins186;1846delT
11 human 50640 PNPLA8 patatin like phospholipase ... XR_001744795.2 64.5% (many diffs)
12 human 50640 PNPLA8 patatin like phospholipase ... XR_001744793.2 57% (many diffs)
13 human 50640 PNPLA8 patatin like phospholipase ... XR_001744797.2 56.7% (many diffs)
14 human 50640 PNPLA8 patatin like phospholipase ... XR_001744796.2 56.1% (many diffs)
15 human 50640 PNPLA8 patatin like phospholipase ... XR_001744798.2 54.3% (many diffs)
16 mouse 67452 Pnpla8 patatin-like phospholipase ... NM_026164.2 84.5% 81.8% (many diffs)
17 mouse 67452 Pnpla8 patatin-like phospholipase ... XM_006516174.3 74.3% 71.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2412
ORF length:
2346
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc tattaatctg actgtagata tatatattta cctccttagt aatgcaagaa
121gtgtttgtgg gaagcagaga agcaagcaac tgtatttctt gttctcacct aagcattact
181ggaggataag ccacatcagt ctacaaagag gttttcatac aaacataata agatgtaaat
241ggaccaaaag tgaagcacat tcttgcagta agcactgtta ctctccaagc aaccatggtt
301tacatattgg gattttgaaa cttagcactt ctgctcccaa gggacttaca aaagtgaaca
361tttgtatgtc ccgtattaaa agtactttga actctgtttc aaaggctgtt tttggcaatc
421aaaatgaaat gatttcacgt ttagctcaat ttaagccaag ttcccaaatt ttaagaaaag
481tatcggatag tggctggtta aaacagaaaa acatcaaaca agccatcaaa tctctgaaaa
541aatatagtga caaatcagca gaaaagagtc cttttccaga agagaaaagt cacattatag
601acaaagaaga agatataggt aaacgcagtc tttttcatta cacaagttct ataaccacaa
661aatttggaga ctcattctac tttttatcaa atcatattaa ttcatatttc aaacgtaagg
721aaaaaatgtc tcaacaaaag gaaaatgaac atttccggga caaatcagaa cttgaagata
781aaaaggtaga agaggggaaa ttaagatctc cagatcctgg catcctggct tataagccag
841gctcagaatc tgtacatacg gtggacaagc ctacaagtcc ttctgcgata cctgatgttc
901ttcaagtttc aactaaacaa agtattgcta actttctttc tcgtcccacg gaaggtgtac
961aagctttagt aggtggttat attggtggac ttgtccccaa attaaagtat gattcaaaga
1021gtcagtcaga agaacaggaa gagcctgcta aaactgatca ggctgtcagc aaagacagaa
1081atgcagagga gaaaaagcgt ttatctcttc agcgagaaaa gattatcgca agggtgagta
1141ttgataacag gacccgggca ttagttcagg cattaagaag aacaactgac ccaaagctct
1201gcattactag ggttgaagaa ctgacttttc atcttctaga atttcctgaa ggaaaaggag
1261tggctgtcaa ggaaagaatt attccatatt tattacgact gagacaaatt aaggatgaaa
1321ctcttcaggc tgcagttaga gaaattttgg ccctaattgg ctatgtggat ccagtgaaag
1381ggagaggaat ccgaattctc tcaattgatg gtggaggaac aaggggcgtg gttgctctcc
1441agaccctacg aaaattagtt gaacttactc agaagccagt tcatcagctc tttgattaca
1501tttgtggtgt aagcacaggt gccatattag ctttcatgtt ggggttgttt catatgccct
1561tggatgaatg tgaggaactt tatcgaaaat taggatcaga tgtattttca caaaatgtca
1621ttgttggaac agtaaaaatg agttggagcc atgcatttta tgacagtcaa acatgggaaa
1681acattcttaa ggataggatg ggatctgcac tgatgattga aacagcaaga aaccccacat
1741gtcctaaggt agctgctgta agtaccatag taaatagagg gataacaccc aaagcttttg
1801tgttcagaaa ctatggtcat tttcctggaa tcaactctca ttatttggga ggctgtcagt
1861ataaaatgtg gcaggccatt agagcctcat ctgctgctcc aggctacttt gcagaatatg
1921cattgggaaa tgatcttcat caagatggag gtttgcttct gaataaccct tcggcattag
1981ctatgcatga gtgtaaatgt ctttGGCCAG ATGTGCCGTT AGAGTGCATA GTATCCCTGG
2041GCACTGGACG TTATGAGAGT GATGTGAGAA ACACGGTAAC ATACACAAGC TTGAAAACTA
2101AACTTTCTAA TGTTATCAAC AGTGCTACAG ATACAGAAGA AGTCCATATA ATGCTTGATG
2161GCCTGTTACC TCCTGACACC TATTTTAGAT TCAATCCTGT AATGTGTGAA AACATACCTC
2221TAGATGAAAG TCGAAATGAA AAGCTGGATC AGCTGCAGTT GGAAGGGTTG AAATACATAG
2281AAAGAAATGA ACAAAAAATG AAAAAAGTTG CAAAAATATT AAGTCAAGAA AAAACAACTC
2341TGCAGAAAAT TAATGATTGG ATAAAATTAA AAACTGATAT GTATGAAGGA CTTCCATCTT
2401TTCAAAATTG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA
2461CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC
2521GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGGG GTTTGCTCTT CAAAACCCAT
2581ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)