Construct: ORF TRCN0000466498
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012479.1_s317c1
- Derived from:
- ccsbBroadEn_12381
- DNA Barcode:
- CCGACGGTGTTTCAAGTGTTGATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COL20A1 (57642)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466498
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57642 | COL20A1 | collagen type XX alpha 1 chain | XM_011528940.1 | 11.9% | 11.9% | 1_3426del |
| 2 | human | 57642 | COL20A1 | collagen type XX alpha 1 chain | XM_011528939.1 | 11.6% | 11.6% | 1_3528del |
| 3 | human | 57642 | COL20A1 | collagen type XX alpha 1 chain | NM_020882.3 | 11.5% | 11.4% | 1_3405del;3613_3614insGTGAGTCTGCCATTCAGA |
| 4 | human | 57642 | COL20A1 | collagen type XX alpha 1 chain | XM_011528938.1 | 11.1% | 10.1% | (many diffs) |
| 5 | human | 57642 | COL20A1 | collagen type XX alpha 1 chain | XM_011528937.1 | 9.1% | 5.9% | 1_3507del;3666_3667ins67;3906_4287del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 531
- ORF length:
- 465
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag aggcctggag ggaactgctg gcctgcctgg accccctggc cccagggggt 121 tccagggcat ggcaggggcc aggggcacta gtggagagcg aggacctcca gggaccgtgg 181 ggccCACAGG ACTGCCAGGG CCCAAAGGGG AACGAGGAGA GAAGGGCGAG CCGCAGTCCC 241 TTGCCACCCT CTACCAGCTT GTGAGCCAGG CCTGTGAGTC TGCCATTCAG ACACACGTGT 301 CAAAGTTCGA CTCCTTCCAC GAGAACACCA GGCCCCCCAT GCCCATCTTG GAGCAGAAGC 361 TGGAGCCGGG CACTGAGCCC CTGGGGTCCC CTGGCACCCG CAGCAAGGCC CTGGTTCCTG 421 GAGAATGGGG GCGTGGTGGC CGCCACCTTG AGGGCAGAGG GGAGCCTGGA GCTGTTGGTC 481 AGATGGGCAG CCCTGGGCAG CAGGGGGCTA GCACCCAGGG CCTCTGGGAG TGCCCAACTT 541 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 601 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 661 CTTGTGGAAA GGACGACCGA CGGTGTTTCA AGTGTTGATG ACGCGTTAAG TCgacaatca 721 acctctggat tacaaaattt gtgaaagatt