Construct: ORF TRCN0000466518
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003074.1_s317c1
- Derived from:
- ccsbBroadEn_01856
- DNA Barcode:
- AAGGCACCCCCTATCAGGACCTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SHOC2 (8036)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466518
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8036 | SHOC2 | SHOC2 leucine rich repeat s... | NM_001324336.1 | 100% | 100% | |
| 2 | human | 8036 | SHOC2 | SHOC2 leucine rich repeat s... | NM_001324337.1 | 100% | 100% | |
| 3 | human | 8036 | SHOC2 | SHOC2 leucine rich repeat s... | NM_007373.3 | 100% | 100% | |
| 4 | human | 8036 | SHOC2 | SHOC2 leucine rich repeat s... | NM_001269039.2 | 92% | 92% | 702_703ins138 |
| 5 | human | 8036 | SHOC2 | SHOC2 leucine rich repeat s... | NR_136749.1 | 30.3% | (many diffs) | |
| 6 | mouse | 56392 | Shoc2 | soc-2 (suppressor of clear)... | NM_001168505.1 | 91.9% | 98.4% | (many diffs) |
| 7 | mouse | 56392 | Shoc2 | soc-2 (suppressor of clear)... | NM_019658.6 | 91.9% | 98.4% | (many diffs) |
| 8 | mouse | 56392 | Shoc2 | soc-2 (suppressor of clear)... | XM_006527228.3 | 91.9% | 98.4% | (many diffs) |
| 9 | mouse | 56392 | Shoc2 | soc-2 (suppressor of clear)... | XM_011247299.2 | 91.9% | 98.4% | (many diffs) |
| 10 | mouse | 56392 | Shoc2 | soc-2 (suppressor of clear)... | XM_017318260.1 | 91.9% | 98.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1812
- ORF length:
- 1746
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tagtagttta ggaaaagaaa aagactctaa agaaaaagat cccaaagtac 121 catcagccaa ggaaagagaa aaggaggcaa aagcctctgg aggttttggg aaagagagca 181 aagaaaaaga acctaagacc aaagggaaag atgccaaaga tggaaagaag gactccagtg 241 ctgcccaacc aggggtggca ttttcagttg acaatacgat caaacggcca aacccagcac 301 ctgggactag aaaaaaatcc agcaatgcag aggtgattaa agagctcaac aaatgccggg 361 aagagaattc aatgcgtttg gacttatcca agagatctat acacatattg ccatcatcaa 421 tcaaagagtt gactcaatta acagaacttt atttatacag taacaaattg cagtccctcc 481 cagcagaggt gggatgttta gtaaatctca tgacactggc tctaagtgaa aattcactta 541 ccagtttgcc tgactctctt gataacttga agaagctgcg gatgcttgat ttacggcata 601 ataaactgag agaaattcct tcagtggtgt ataggctgga ttctctcacc actctttacc 661 ttcgctttaa tcgtataact actgtggaaa aggacatcaa aaacttgtca aaactcagca 721 tgcttagcat tcgagagaac aaaattaaac aactacctgc tgaaattggt gaattatgta 781 acctcattac gctggatgta gctcacaatc aacttgaaca ccttccaaag gagattggaa 841 actgtacaca gataaccaac cttgacttgc agcacaatga actgctagac ctcccagata 901 ctataggaaa cctgtccagt ttaagtcgtc ttggtctgag atataacaga ctgtcagcaa 961 tacccagatc attagcaaaa tgcagtgcac ttgaagaatt aaatttagag aacaataaca 1021 tttctacttt accagagagt cttttatcaa gtcttgtgaa actgaatagt ttgaccttag 1081 ctagaaattg cttccagttg tatccagtgg gtggtccatc tcagttttct accatctatt 1141 ccctcaacat ggaacacaat cgaatcaaca aaattccatt tggaattttc tccagagcaa 1201 aagtattaag taagctgaat atgaaggaca atcagttaac atcacttccc ttggattttg 1261 gaacttggac cagtatggta gaattgaatt tagccactaa tcagctcaca aagatccctg 1321 aggatgtgtc tggtctcgtt tctcttgagg ttcttatctt atcaaacaat cttctaaaga 1381 agcttcccca tggtcttgga aaccttagga agttaagaga gttGGATCTA GAAGAGAACA 1441 AATTGGAATC CTTGCCAAAT GAAATTGCAT ATCTTAAGGA TTTACAGAAA TTAGTCTTGA 1501 CAAACAACCA GTTGACCACT CTTCCCAGAG GCATTGGTCA CCTTACTAAT CTCACACATC 1561 TGGGCCTTGG AGAGAACCTA CTTACTCACC TTCCTGAAGA AATTGGTACA CTGGAGAACC 1621 TAGAAGAACT GTATTTGAAT GACAACCCCA ACCTGCATAG CCTTCCCTTT GAGCTGGCAC 1681 TCTGCAGCAA GCTTTCAATC ATGAGTATTG AGAACTGTCC ACTCAGTCAC CTTCCACCTC 1741 AGATTGTTGC TGGGGGGCCT TCTTTCATCA TTCAGTTCTT AAAGATGCAG GGTCCATATC 1801 GTGCCATGGT CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1861 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1921 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAG GCACCCCCTA TCAGGACCTC 1981 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t