Construct: ORF TRCN0000466530

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009477.1_s317c1
Derived from:
ccsbBroadEn_00030
DNA Barcode:
CTAGACATACATGATGCGGCACCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ADORA2B (136)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466530
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 136 ADORA2B adenosine A2b receptor NM_000676.2 100% 100%
2 human 136 ADORA2B adenosine A2b receptor XM_011523659.3 67.9% 67.1% (many diffs)
3 human 136 ADORA2B adenosine A2b receptor XM_017024197.2 67.9% 66.2% (many diffs)
4 human 136 ADORA2B adenosine A2b receptor XR_001752428.1 43.1% 1_523del;858_1237del;1900_2306del
5 human 136 ADORA2B adenosine A2b receptor XM_011523661.2 34.3% 33.7% (many diffs)
6 mouse 11541 Adora2b adenosine A2b receptor NM_007413.4 87.4% 87.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1062
ORF length:
996
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgct gctggagaca caggacgcgc tgtacgtggc gctggagctg gtcatcgccg
121cgctttcggt ggcgggcaac gtgctggtgt gcgccgcggt gggcacggcg aacactctgc
181agacgcccac caactacttc ctggtgtccc tggctgcggc cgacgtggcc gtggggctct
241tcgccatccc ctttgccatc accatcagcc tgggcttctg cactgacttc tacggctgcc
301tcttcctcgc ctgcttcgtg ctggtgctca cgcagagctc catcttcagc cttctggccg
361tggcagtcga cagatacctg gccatctgtg tcccgctcag gtataaaagt ttggtcacgg
421ggacccgagc aagaggggtc attgctgtcc tctgggtcct tgcctttggc atcggattga
481ctccattcct ggggtggaac agtaaagaca gtgccaccaa caactgcaca gaaccctggg
541atggaaccac gaatgaaagc tgctgccttg tgaagtgtct ctttgagaat gtggtcccca
601tgagctacat ggtatatttc aatttctttg ggtgtgttct gcccccactg cttataatgc
661tggtgatcta cattaagatc ttcctggtgg cctgcaggca gcttcagcgc actgagctga
721tggaccactc gaggaccacc cTCCAGCGGG AGATCCATGC AGCCAAGTCA CTGGCCATGA
781TTGTGGGGAT TTTTGCCCTG TGCTGGTTAC CTGTGCATGC TGTTAACTGT GTCACTCTTT
841TCCAGCCAGC TCAGGGTAAA AATAAGCCCA AGTGGGCAAT GAATATGGCC ATTCTTCTGT
901CACATGCCAA TTCAGTTGTC AATCCCATTG TCTATGCTTA CCGGAACCGA GACTTCCGCT
961ACACTTTTCA CAAAATTATC TCCAGGTATC TTCTCTGCCA AGCAGATGTC AAGAGTGGGA
1021ATGGTCAGGC TGGGGTACAG CCTGCTCTCG GTGTGGGCCT ATGCCCAACT TTCTTGTACA
1081AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1141AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1201AGGACGACTA GACATACATG ATGCGGCACC TACGCGTTAA GTCgacaatc aacctctgga
1261ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)