Construct: ORF TRCN0000466532
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018969.3_s317c1
- Derived from:
- ccsbBroadEn_14877
- DNA Barcode:
- GTCACTGTGTGTTCAAAAGAATTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- VRK2 (7444)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466532
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001130480.2 | 98.6% | 48.3% | (many diffs) |
| 2 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001130481.2 | 98.6% | 48.3% | (many diffs) |
| 3 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001288837.1 | 98.6% | 48.3% | (many diffs) |
| 4 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_006296.6 | 98.6% | 48.3% | (many diffs) |
| 5 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_005264540.4 | 98.6% | 48.3% | (many diffs) |
| 6 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_011533092.3 | 98.6% | 48.3% | (many diffs) |
| 7 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001130482.2 | 94.2% | 43.8% | (many diffs) |
| 8 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_006712090.4 | 76.6% | 61.8% | (many diffs) |
| 9 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004858.2 | 76.6% | 61.8% | (many diffs) |
| 10 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001130483.2 | 76.5% | 61.9% | (many diffs) |
| 11 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001288838.1 | 76.5% | 61.9% | (many diffs) |
| 12 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_006712091.4 | 76.5% | 61.9% | (many diffs) |
| 13 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001288836.1 | 75.6% | 25.1% | (many diffs) |
| 14 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | NM_001288839.1 | 75.6% | 25.1% | (many diffs) |
| 15 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_006712092.4 | 75.6% | 25.1% | (many diffs) |
| 16 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_006712093.4 | 75.6% | 25.1% | (many diffs) |
| 17 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004859.2 | 75.6% | 25.1% | (many diffs) |
| 18 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004860.2 | 75.6% | 25.1% | (many diffs) |
| 19 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004861.2 | 75.6% | 25.1% | (many diffs) |
| 20 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004862.2 | 75.6% | 25.1% | (many diffs) |
| 21 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004863.2 | 75.6% | 25.1% | (many diffs) |
| 22 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004865.1 | 53.4% | 32.2% | (many diffs) |
| 23 | human | 7444 | VRK2 | VRK serine/threonine kinase 2 | XM_017004866.1 | 53.4% | 32.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 831
- ORF length:
- 765
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc accaaaaaga aatgaaaaat acaaacttcc tattccattt ccagaaggca 121 aggttctgga tgatatggaa ggcaatcagt gggtactggg caagaagatt ggctctggag 181 gatttggatt gatatattta gctttcccca caaataaacc agagaaagat gcaagacatg 241 tagtaaaagt ggaatatcaa gaaaatggcc cgttattttc agaacttaaa ttttatcaga 301 gagttgcaaa aaaagactgt atcaaaaagt ggatagaacg caaacaactt gattatttag 361 gaattcctct gttttatgga tctggtctga ctgaattcaa gggaagaagt tacagattta 421 tggtaatgga aagactagga atagatttac agaagatctc aggccagaat ggtaccttta 481 aaaagtcaac tgtcctgcaa ttaggtatcc gaatgttgga tgtactggaa tatatacatg 541 aaaatgaata tgttcatggt gatataaaag cagcaaatct acttttgggt tacaaaaatc 601 cagaccaggt ttatcttgca gattatggac tttcctacag atattgtccc aatgggaacc 661 acaaacagta tcaggaaaat cctagaaaag gccataatgg gacaatagag tttaccagct 721 tggatgccca caagggagta gccttgtcca gacgaagtga cgttgagatc ctcggctact 781 gcatgctggg ggtgnntggg tgtgggaaac ttcccttggg gaacagaacc tgaaggaccc 841 ctgtggctgt gcagactgct aaaaacaaat tcttgttgga cgagctcccc cagtcagtgc 901 ttaatnggct ccctctggaa gcagtgnctg tgaaatagcc caatttttgg tatgtgctca 961 tagtttagca tatgatgaaa agccaaacta tcaagccctc aagaaaattt tgaaccctca 1021 tggaatacct ttaggaccac tggacttttc cacaaaagga cagagtataa atgtccatac 1081 tccaaacagt caaaaagttg attcacaaaa ggctgcaaca aagcaagtca acaaggcaca 1141 caataggtta atcgaaaaaa aagtccacag tgagagaagc gctgagtcct gtgcaacatg 1201 gaaagtgcag aaagaggaga aactgattgg attgatgaac aatgaagcag cTCAGGAAAG 1261 CACAAGGAGA AGACAGAAAT ATCAAGAGTC TCAAGAACCT TTGAATGAAG TAAACAGTTT 1321 CCCACAAAAA ATCAGCTATA CACAATTCCC AAACTCATTT TATGAGCCTC ATCAAGATTT 1381 TACCAGTCCA GATATATTCA AGAAGTCAAG ATCTCCATCT TGGTATAAAT ACACTTCCAC 1441 AGTCAGCACG GGGATCACAG ACTTAGAAAG TTCAACTGGA CTTTGGCCTA CAATTTCCCA 1501 GTTTACTCTT AGTGAAGAGA CAAACGCAGA TGTTTATTAT TATCGCATCA TCATACCTGT 1561 CCTTTTGATG TTAGTATTTC TTGCTTTATT TTTTCTCTTG CCAACTTTCT TGTACAAAGT 1621 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 1681 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 1741 CGAGTCACTG TGTGTTCAAA AGAATTAACG CGTTAAGTCg acaatcaacc tctggattac 1801 aaaatttgtg aaagatt