Construct: ORF TRCN0000466551
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003744.1_s317c1
- Derived from:
- ccsbBroadEn_08634
- DNA Barcode:
- ATCAAAGATACCAGTGTTATTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSDMC (56169)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466551
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56169 | GSDMC | gasdermin C | NM_031415.3 | 99.8% | 99.6% | 67C>T;1424T>C;1500G>A |
| 2 | human | 56169 | GSDMC | gasdermin C | XM_011517158.2 | 98% | 97.6% | (many diffs) |
| 3 | human | 56169 | GSDMC | gasdermin C | XM_011517159.2 | 96% | 95.8% | (many diffs) |
| 4 | human | 56169 | GSDMC | gasdermin C | XM_011517161.2 | 90.4% | 85.4% | (many diffs) |
| 5 | human | 56169 | GSDMC | gasdermin C | XM_011517162.2 | 85.6% | 85.2% | (many diffs) |
| 6 | human | 56169 | GSDMC | gasdermin C | XM_011517163.3 | 82.9% | 82.8% | 0_1ins258;1166T>C;1242G>A |
| 7 | human | 56169 | GSDMC | gasdermin C | XM_017013645.1 | 70.5% | 63.3% | (many diffs) |
| 8 | human | 56169 | GSDMC | gasdermin C | XR_928341.2 | 57.1% | (many diffs) | |
| 9 | human | 56169 | GSDMC | gasdermin C | XM_011517165.1 | 53.1% | 52.5% | (many diffs) |
| 10 | human | 56169 | GSDMC | gasdermin C | XR_928340.2 | 52.3% | (many diffs) | |
| 11 | human | 56169 | GSDMC | gasdermin C | XM_011517166.3 | 51.4% | 51.3% | 0_1ins738;686T>C;762G>A |
| 12 | human | 56169 | GSDMC | gasdermin C | XR_928339.2 | 50.5% | (many diffs) | |
| 13 | human | 56169 | GSDMC | gasdermin C | XM_011517167.2 | 49.6% | 47.7% | (many diffs) |
| 14 | human | 56169 | GSDMC | gasdermin C | XM_011517168.2 | 49.3% | 47.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1590
- ORF length:
- 1524
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ctccatgttg gaacgcatta gcaaaaattt ggtcaaagag attggaagca 121 aagacctgac atctgtcaaa tacctattga gtgccaccaa attacgtcag tttgttatat 181 tacgaaagaa gaaggattct cgttcatcat tttgggaaca atctgactat gttccagttg 241 aattctccct caatgacatc ctggagccaa gttcttcagt cctagaaact gttgtgacag 301 gaccgttcca cttcagtgac attatgatcc agaagcataa ggctgacatg ggtgtgaatg 361 ttggtataga agtgagtgtg tcaggggagg cctctgtgga ccatggatgc tccctcgagt 421 ttcaaattgt taccatccca tcaccaaacc tggaagactt tcaaaaaagg aaactgttgg 481 atccagagcc atcatttctg aaggagtgcc ggaggagagg ggacaacctg tacgtggtga 541 cagaggctgt tgaactgatc aacaatactg tgctgtacga tagcagtagt gtgaatattt 601 tagggaaaat tgctctttgg attacctatg gcaagggtca aggccaagga gagagtctca 661 gagtgaagaa gaaggcgctg actcttcaga aaggcatggt gatggcttat aagagaaagc 721 agctggttat caaggagaaa gccattctca tctcagatga tgatgaacag agaacctttc 781 aagatgagta cgaaatttcc gaaatggtag gctactgtgc tgcgaggagt gaggggttgc 841 taccatcatt tcataccatc tctccaaccc tcttcaatgc ctcatccaat gatatgaagt 901 taaaaccaga gctatttctg acacagcaat ttttgagcgg gcatttgcca aaatacgaac 961 aagttcacat cctcccagta ggaagaatag aggaaccctt ctggcaaaat ttcaagcatc 1021 tacaagagga ggttttccag aaaataaaga cactggctca gctctcaaag gatgttcagg 1081 atgtcatgtt ctacagtatc ctggccatgc tcagagacag aggggctcta caggacctga 1141 tgaacatgct ggaattggac agctcaggtc atttggatgg ccctggtggt gccatcctaa 1201 agaaacttca acaggattca aaccatgcat ggtttaaccc aaaggacccc attctttatc 1261 tccttGAAGC CATAATGGTG CTGAGTGACT TCCAACACGA TTTGCTGGCC TGTTCCATGG 1321 AGAAGAGGAT CCTGCTTCAG CAACAGGAGC TGGTAAGGAG CATCCTGGAG CCAAACTTCA 1381 GATACCCCTG GAGCATTCCC TTCACCCTCA AACCTGAGCT CCTCGCCCCA CTCCAGAGTG 1441 AGGGTTTGGC CATCACCTAT GGCCTGCTGG AGGAGTGTGG CCTTAGGACG GAGCTGGATA 1501 ACCCCAGGTC AACCTGGGAT GTAGAAGCAA AGATGCCCCT GTCTGCCCTC TATGGGACTC 1561 TCTCATTGCT GCAGCAGCTG GCTGAGGCCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1621 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1681 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATCAA 1741 AGATACCAGT GTTATTCCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1801 tgaaagatt