Construct: ORF TRCN0000466559
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017514.1_s317c1
- Derived from:
- ccsbBroadEn_09608
- DNA Barcode:
- CCCCAAAAACTATTTTTTATTCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM86A (144110)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466559
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144110 | TMEM86A | transmembrane protein 86A | NM_153347.3 | 99.8% | 99.5% | 710C>A |
| 2 | mouse | 67893 | Tmem86a | transmembrane protein 86A | NM_026436.3 | 86.9% | 88.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 786
- ORF length:
- 720
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gtccccggtc actgtggtga agagtgaagg acccaaactg gtgccgttct 121 tcaaggccac ctgcgtgtat tttgtgctct ggctgccctc atctagccca tcgtgggtca 181 gcaccctcat caagtgcctg cctatcttct gcctctggct cttccttctg gcccatggcc 241 tgggattcct gctggcccac cccagcgcca cccgcatctt tgtggggctt gtcttctctg 301 ctgtaggtga cgccttcctc atctggcagg accaaggata cttcgtgcat ggtctgctga 361 tgtttgctgt gacccacatg ttctacgcct cggcctttgg catgcagcca ctggctcTTC 421 GGACAGGTCT GGTGATGGCA GCGCTGTCGG GCCTGTGCTA TGCCCTCCTC TACCCATGCC 481 TCTCAGGTGC CTTCACCTAC CTGGTGGGGG TCTATGTGGC CCTTATCGGC TTCATGGGCT 541 GGCGAGCTAT GGCAGGGCTG CGGCTGGCCG GGGCAGACTG GCGCTGGACA GAGCTGGCAG 601 CTGGCAGTGG TGCACTCTTC TTTATCATCT CAGACCTGAC CATCGCCCTC AACAAATTCT 661 GTTTTCCTGT GCCCTACTCT CGGGCGCTTA TCATGTCCAC CTACTATGTG GCCCAGATGC 721 TCGTCGCCTT GTCAGCTGTC GAAAGCCGGG AGCCTGTGGA ACACTACAGA CTGAACAAGG 781 CCAACTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 841 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 901 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCCCAAAAA CTATTTTTTA TTCCAACGCG 961 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt