Construct: ORF TRCN0000466575
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011203.1_s317c1
- Derived from:
- ccsbBroadEn_00893
- DNA Barcode:
- AAACTAGCCTCATCAAAATCACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCND2 (3751)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466575
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3751 | KCND2 | potassium voltage-gated cha... | NM_012281.3 | 100% | 100% | |
| 2 | human | 3751 | KCND2 | potassium voltage-gated cha... | XM_011516165.3 | 59.3% | 58.5% | (many diffs) |
| 3 | mouse | 16508 | Kcnd2 | potassium voltage-gated cha... | NM_019697.3 | 88.8% | 99% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1959
- ORF length:
- 1890
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcgggg gtggcagcgt ggctgccttt tgcaagggca gcggctatcg 121 ggtggatgcc tgtggcctcg gggcctatgc cggctccccc gaggcaggag aggaaaagga 181 cccaagatgc tctcattgtg ctgaatgtga gtggcacccg cttccagacg tggcaggaca 241 ccctggaacg ttacccagac actctactgg gcagttctga gagggacttt ttctaccacc 301 cagaaactca gcagtatttc tttgaccgtg acccagacat cttccgccac atcctgaatt 361 tctaccgcac tgggaagctc cactatcctc gccacgagtg catctctgct tacgatgaag 421 aactggcctt ctttggcctc atcccggaaa tcatcggcga ctgctgttat gaggagtaca 481 aggatcgcag gcgagagaac gccgagcgcc tgcaggacga cgcggatacc gacaccgctg 541 gggagagcgc cttgcccacc atgactgcaa ggcagagggt ctggagggcc ttcgagaacc 601 cccacaccag cacgatggcc ctggtgttct actatgtcac ggggtttttc attgccgtct 661 ctgtcatcgc gaatgtggtg gaaacagtgc cgtgcggatc aagcccaggt cacattaaag 721 aactgccctg tggagagcgg tatgctgtgg ccttcttctg cttggacacg gcctgcgtca 781 tgatcttcac agttgagtat ttgcttcgcc tggctgcagc gcctagtcgt taccgttttg 841 tgcgtagtgt catgagtatc atcgacgtgg tggccatcct gccttattac attgggctgg 901 tgatgacaga caatgaggac gtcagcggag cctttgtcac actccgagtc ttccgggtct 961 tcaggatctt taagttttcc cgccactctc aaggcctgcg catcctgggg tacacactga 1021 agagttgtgc ctcagaattg ggcttcttgc ttttctcgct caccatggct atcatcatct 1081 tcgctacagt tatgttctac gcagagaagg ggtcttcggc tagcaagttc accagcatcc 1141 ctgcagcctt ctggtatacc atcgtcacca tgacaacact agggtatggt gacatggtgc 1201 caaaaaccat agcagggaag atttttggtt ctatctgttc gctgagtggg gtcttggtca 1261 ttgctctacc tgttccggtg attgtatcca acttcagtcg catctaccac cagaatcaac 1321 gagcagacaa acgaagggca caaaagaaag ctagactggc caggatccgg gcagccaaaa 1381 gcggaagcgc aaatgcttac atgcagagca aacggaatgg tttactcagt aatcagctgc 1441 agtcctcaga ggatgagcag gcttttgtta gcaaatccgg ctccagcttt gaaacccagc 1501 accaccacct gcttcactgc ctggaaaaaa ccacgaatca cgagtttgtg gacgaacaag 1561 tctttgaaga aagctgcatg gaagttgcaa ctgttaatcg tccttcaagt cacagtcctt 1621 cactgtcttc acaacaagga gtcaccagca ccTGCTGTTC ACGACGACAC AAAAAAACTT 1681 TTCGCATCCC AAATGCCAAT GTATCAGGAA GCCATCAAGG TAGTATACAA GAACTCAGCA 1741 CGATTCAGAT CAGATGTGTG GAGAGAACAC CTCTGTCTAA CAGCCGATCC AGTTTAAATG 1801 CCAAAATGGA AGAGTGTGTT AAACTAAACT GTGAACAACC TTATGTGACT ACAGCAATAA 1861 TAAGCATCCC AACACCTCCA GTAACCACAC CAGAAGGAGA CGATAGGCCA GAATCCCCTG 1921 AGTACTCAGG AGGAAATATT GTCAGAGTTT CTGCTTTGTT GCCAACTTTC TTGTACAAAG 1981 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2041 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2101 ACGAAAACTA GCCTCATCAA AATCACTAAC GCGTTAAGTC gacaatcaac ctctggatta 2161 caaaatttgt gaaagatt