Construct: ORF TRCN0000466581
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018412.1_s317c1
- Derived from:
- ccsbBroadEn_00305
- DNA Barcode:
- AGGTATATGTAACCTTGCGGCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRM3 (1131)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466581
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1131 | CHRM3 | cholinergic receptor muscar... | NM_000740.3 | 100% | 100% | |
| 2 | human | 1131 | CHRM3 | cholinergic receptor muscar... | NM_001347716.2 | 100% | 100% | |
| 3 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_005273032.3 | 100% | 100% | |
| 4 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_011544041.2 | 100% | 100% | |
| 5 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_011544043.2 | 100% | 100% | |
| 6 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_011544044.2 | 100% | 100% | |
| 7 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_011544046.2 | 100% | 100% | |
| 8 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_011544047.2 | 100% | 100% | |
| 9 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000152.2 | 100% | 100% | |
| 10 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000153.1 | 100% | 100% | |
| 11 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000154.1 | 100% | 100% | |
| 12 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000155.1 | 100% | 100% | |
| 13 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000156.1 | 100% | 100% | |
| 14 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000157.2 | 100% | 100% | |
| 15 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000158.1 | 100% | 100% | |
| 16 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000159.1 | 100% | 100% | |
| 17 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000160.2 | 100% | 100% | |
| 18 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000161.1 | 100% | 100% | |
| 19 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000162.1 | 100% | 100% | |
| 20 | human | 1131 | CHRM3 | cholinergic receptor muscar... | XM_017000163.2 | 100% | 100% | |
| 21 | mouse | 12671 | Chrm3 | cholinergic receptor, musca... | NM_033269.4 | 86.9% | 91.6% | (many diffs) |
| 22 | mouse | 12671 | Chrm3 | cholinergic receptor, musca... | XM_006516468.3 | 86.9% | 91.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1839
- ORF length:
- 1770
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccttgcac aataacagta caacctcgcc tttgtttcca aacatcagct 121 cctcctggat acacagcccc tccgatgcag ggctgccccc gggaaccgtc actcatttcg 181 gcagctacaa tgtttctcga gcagctggca atttctcctc tccagacggt accaccgatg 241 accctctggg aggtcatacc gtctggcaag tggtcttcat cgctttctta acgggcatcc 301 tggccttggt gaccatcatc ggcaacatcc tggtaattgt gtcatttaag gtcaacaagc 361 agctgaagac ggtcaacaac tacttcctct taagcctggc ctgtgccgat ctgattatcg 421 gggtcatttc aatgaatctg tttacgacct acatcatcat gaatcgatgg gccttaggga 481 acttggcctg tgacctctgg cttgccattg actacgtagc cagcaatgcc tctgttatga 541 atcttctggt catcagcttt gacagatact tttccatcac gaggccgctc acgtaccgag 601 ccaaacgaac aacaaagaga gccggtgtga tgatcggtct ggcttgggtc atctcctttg 661 tcctttgggc tcctgccatc ttgttctggc aatactttgt tggaaagaga actgtgcctc 721 cgggagagtg cttcattcag ttcctcagtg agcccaccat tacttttggc acagccatcg 781 ctgcttttta tatgcctgtc accattatga ctattttata ctggaggatc tataaggaaa 841 ctgaaaagcg taccaaagag cttgctggcc tgcaagcctc tgggacagag gcagagacag 901 aaaactttgt ccaccccacg ggcagttctc gaagctgcag cagttacgaa cttcaacagc 961 aaagcatgaa acgctccaac aggaggaagt atggccgctg ccacttctgg ttcacaacca 1021 agagctggaa acccagctcc gagcagatgg accaagacca cagcagcagt gacagttgga 1081 acaacaatga tgctgctgcc tccctggaga actccgcctc ctccgacgag gaggacattg 1141 gctccgagac gagagccatc tactccatcg tgctcaagct tccgggtcac agcaccatcc 1201 tcaactccac caagttaccc tcatcggaca acctgcaggt gcctgaggag gagctgggga 1261 tggtggactt ggagaggaaa gccgacaagc tgcaggccca gaagagcgtg gacgatggag 1321 gcagttttcc aaaaagcttc tccaagcttc ccatccagct agagtcagcc gtggacacag 1381 ctaagacttc tgacgtcaac tcctcagtgg gtaagagcac ggccactcta cctctgtccT 1441 TCAAGGAAGC CACTCTGGCC AAGAGGTTTG CTCTGAAGAC CAGAAGTCAG ATCACTAAGC 1501 GGAAAAGGAT GTCCCTGGTC AAGGAGAAGA AAGCGGCCCA GACCCTCAGT GCGATCTTGC 1561 TTGCCTTCAT CATCACTTGG ACCCCATACA ACATCATGGT TCTGGTGAAC ACCTTTTGTG 1621 ACAGCTGCAT ACCCAAAACC TTTTGGAATC TGGGCTACTG GCTGTGCTAC ATCAACAGCA 1681 CCGTGAACCC CGTGTGCTAT GCTCTGTGCA ACAAAACATT CAGAACCACT TTCAAGATGC 1741 TGCTGCTGTG CCAGTGTGAC AAAAAAAAGA GGCGCAAGCA GCAGTACCAG CAGAGACAGT 1801 CGGTCATTTT TCACAAGCGC GCACCCGAGC AGGCCTTGTT GCCAACTTTC TTGTACAAAG 1861 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1921 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1981 ACGAAGGTAT ATGTAACCTT GCGGCCAAAC GCGTTAAGTC gacaatcaac ctctggatta 2041 caaaatttgt gaaagatt