Transcript: Mouse XM_006516468.3

PREDICTED: Mus musculus cholinergic receptor, muscarinic 3, cardiac (Chrm3), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Chrm3 (12671)
Length:
4357
CDS:
847..2616

Additional Resources:

NCBI RefSeq record:
XM_006516468.3
NBCI Gene record:
Chrm3 (12671)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006516468.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000027341 GTGGCATTTAAGGTCAACAAA pLKO.1 1114 CDS 100% 5.625 7.875 N Chrm3 n/a
2 TRCN0000027397 CTATATCAACAGCACCGTGAA pLKO.1 2442 CDS 100% 4.050 5.670 N Chrm3 n/a
3 TRCN0000011261 CCGAGCCAAACGAACAACAAA pLKO.1 1371 CDS 100% 5.625 4.500 N CHRM3 n/a
4 TRCN0000027331 CCTCTGGCTTTCCATTGACTA pLKO.1 1269 CDS 100% 4.950 3.465 N Chrm3 n/a
5 TRCN0000027377 CGGAGAATGTTTCATTCAGTT pLKO.1 1497 CDS 100% 4.950 3.465 N Chrm3 n/a
6 TRCN0000027358 CCTCTGGGAATTTCTCCTCAA pLKO.1 980 CDS 100% 4.050 2.430 N Chrm3 n/a
7 TRCN0000011262 AGACCAGAAGTCAGATCACTA pLKO.1 2252 CDS 100% 4.950 3.465 N CHRM3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006516468.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00305 pDONR223 100% 86.9% 91.6% None (many diffs) n/a
2 ccsbBroad304_00305 pLX_304 0% 86.9% 91.6% V5 (many diffs) n/a
3 TRCN0000466581 AGGTATATGTAACCTTGCGGCCAA pLX_317 22.8% 86.9% 91.6% V5 (many diffs) n/a
4 TRCN0000491837 TTTAGGACGTCGCGTTATCACGGT pLX_317 22.9% 86.9% 91.6% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000489844 ATCTCTAGAACCGTATCCGAGGAC pLX_317 22.3% 86.9% 91.6% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000489751 GACCACGGCGGCAACATTCCAGCA pLX_317 22.7% 86.9% 91.5% V5 (many diffs) n/a
7 TRCN0000489887 GAACTTTCCGTTATGTCTCAATCA pLX_317 19.8% 86.9% 91.5% V5 (many diffs) n/a
8 TRCN0000491958 ACCGACTCTGATCATCTCTTGAAT pLX_317 20.5% 86.9% 91.5% V5 (many diffs) n/a
9 TRCN0000488345 CCCTGGTCCGTTTTCGACCTAGAC pLX_317 20% 82.9% 87.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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