Construct: ORF TRCN0000466588
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014608.1_s317c1
- Derived from:
- ccsbBroadEn_06088
- DNA Barcode:
- CAATCAATAACCTCAGTACCACGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- AKR1C1 (1645)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466588
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1645 | AKR1C1 | aldo-keto reductase family ... | NM_001353.6 | 98.6% | 71.2% | (many diffs) |
| 2 | human | 1646 | AKR1C2 | aldo-keto reductase family ... | NM_001354.5 | 96.7% | 69% | (many diffs) |
| 3 | human | 1646 | AKR1C2 | aldo-keto reductase family ... | NM_205845.2 | 96.7% | 69% | (many diffs) |
| 4 | human | 8644 | AKR1C3 | aldo-keto reductase family ... | NM_001253908.1 | 90.8% | 62.5% | (many diffs) |
| 5 | human | 1645 | AKR1C1 | aldo-keto reductase family ... | XM_017015791.2 | 90.7% | 63.1% | (many diffs) |
| 6 | human | 8644 | AKR1C3 | aldo-keto reductase family ... | NM_003739.6 | 90.4% | 62.8% | (many diffs) |
| 7 | human | 1646 | AKR1C2 | aldo-keto reductase family ... | NM_001321027.1 | 88.7% | 60.9% | (many diffs) |
| 8 | human | 1109 | AKR1C4 | aldo-keto reductase family ... | NM_001818.4 | 87.5% | 58.8% | (many diffs) |
| 9 | human | 1646 | AKR1C2 | aldo-keto reductase family ... | NM_001135241.2 | 40.3% | 50.8% | (many diffs) |
| 10 | human | 8644 | AKR1C3 | aldo-keto reductase family ... | NM_001253909.2 | 37.2% | 48.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 765
- ORF length:
- 699
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ttcgaaatat cagtgtgtga agctgaatga tggtcacttc atgcctgtcc 121 tgggatttgg cacctatgcg cctgcagagg ttcctaaaag taaagcttta gaggccacca 181 aattggcaat tgaagctggc ttccgccata ttgattctgc tcatttatac aataatgagg 241 agcaggttgg actggccatc cgaagcaaga ttgcagatgg cagtgtgaag agagaagaca 301 tattctacac ttcaaagctt tggtgcaatt cccatcgacc agagttggtc cgaccagcct 361 tggaaaggtc actgaaaaat cttcaattgg attatgttga cctctacctt attcattttc 421 cagtgtctgt aaagccaggt gaggaagtga tcccaaaaga tgaaaatgga aaaatactat 481 ttgacacagt ggatctctgt gccacgtggg aggccgtgga gaagtgtaaa gatgcaggat 541 tggccaagtc catcggggtg tccaacttca accgcaggca gctggagatg atcctcaaca 601 agccagggct caagtacaag cctgtctgca accaggtgga atgtcatcct tacttcaacc 661 agagaaaact gctggatttc tgcaagtcaa aagacattgt tctggttgcc tatagtgctc 721 tgggatccca ccgagaagaa ccatgggTGA TGCGCTGCTC ATTGTAGCTC TTGGAGGACC 781 CAGTCCTTTG TGCCTTGGCA AAAAAGCACA AGCGAACCCC AGCCCTGATT GCCCTGCGCT 841 ACCAGCTACA GCGTGGGGTT GTGGTCCTGG CCAAGAGCTA CAATGAGCAG CGCATCAGAC 901 AGAACGTGCA GGTGTTTGAA TTCCAGTTGA CTTCAGAGGA GATGAAAGCC ATAGATGGCC 961 TAAACAGAAA TGTGCGATAT TTGACCCTTG ATATTTTTGC TGGCCCCCCT AATTATCCAT 1021 TTTCTGATGA ATATTCCTCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1081 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1141 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CAATCAATAA CCTCAGTACC 1201 ACGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt