Construct: ORF TRCN0000466590
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007130.1_s317c1
- Derived from:
- ccsbBroadEn_05149
- DNA Barcode:
- GCTGGACCTCCCTAGCACTTCTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM252 (169693)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466590
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 169693 | TMEM252 | transmembrane protein 252 | NM_153237.2 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 576
- ORF length:
- 510
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gaacagaact ggcctcattc tctgtgctct tgccctcctg atgggtttcc 121 tgatggtctg cctgggggcc ttcttcattt cctggggctc catattcgac tgtcagggga 181 gcctgattgc ggcctatttg cttctgcctc tggggtttgt gatccttctg agtggaattt 241 TCTGGAGCAA CTATCGCCAG GTGACTGAAA GCAAAGGAGT GTTGAGGCAC ATGCTCCGAC 301 AACACCTTGC TCATGGGGCC CTGCCCGTGG CCACAGTAGA CAGGCCAGAC TTTTACCCTC 361 CAGCTTATGA AGAGAGCCTT GAGGTGGAAA AGCAGAGCTG TCCTGCAGAG AGAGAGGCCT 421 CTGGCATTCC TCCACCTCTA TATACAGAGA CGGGCCTGGA ATTCCAGGAT GGAAATGACT 481 CCCACCCAGA GGCCCCACCA TCTTATAGAG AGTCCATAGC CGGCCTGGTG GTGACAGCAA 541 TCTCAGAGGA CGCCCAGAGG CGAGGCCAAG AGTGCTGCCC AACTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 AGCTGGACCT CCCTAGCACT TCTCGACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt