Construct: ORF TRCN0000466605
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006783.1_s317c1
- Derived from:
- ccsbBroadEn_13073
- DNA Barcode:
- GGGCATGATAGTAGCACCACATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BORCS7 (119032)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466605
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 119032 | BORCS7 | BLOC-1 related complex subu... | NM_001136200.2 | 99% | 99% | 1_3delATG |
| 2 | human | 119032 | BORCS7 | BLOC-1 related complex subu... | NM_144591.4 | 99% | 99% | 1_3delATG |
| 3 | human | 100528007 | BORCS7-ASMT | BORCS7-ASMT readthrough (NM... | NR_037644.1 | 11.5% | 1_80del;396_2732del | |
| 4 | mouse | 66439 | Borcs7 | BLOC-1 related complex subu... | NM_025563.3 | 90.1% | 96.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 405
- ORF length:
- 315
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCGACTGG AACGCCAGAG TCTCAAGCGC 121 GGTTCGGTCA GTCCGTGAAG GGGCTTCTCA CGGAGAAGGT GACCACCTGT GGTACTGACG 181 TAATCGCGCT CACCAAGCAG GTGCTGAAAG GCTCCCGGAG CTCCGAGCTG CTAGGTCAGG 241 CAGCTCGAAA CATGGTACTC CAGGAAGATG CCATCTTGCA CTCAGAAGAT AGTTTAAGGA 301 AGATGGCAAT AATAACAACA CATCTTCAAT ACCAGCAAGA AGCTATTCAG AAGAATGTTG 361 AACAGTCATC GGATCTACAG GACCAGTTGA ATCATCTGTT GAAATACCCA ACTTTCTTGT 421 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 481 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 541 GAAAGGACGA GGGCATGATA GTAGCACCAC ATACACGCGT TAAGTCgaca atcaacctct 601 ggattacaaa atttgtgaaa gatt