Construct: ORF TRCN0000466626
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016691.1_s317c1
- Derived from:
- ccsbBroadEn_00301
- DNA Barcode:
- GAGAGAACCATGAACGTTGCGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHD2 (1106)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466626
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1106 | CHD2 | chromodomain helicase DNA b... | NM_001042572.3 | 100% | 100% | |
| 2 | human | 1106 | CHD2 | chromodomain helicase DNA b... | NM_001271.4 | 27.3% | 27.4% | 1503_5484delinsG |
| 3 | mouse | 244059 | Chd2 | chromodomain helicase DNA b... | NM_001081345.2 | 24.8% | 26.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1569
- ORF length:
- 1503
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gagaaataag gacaaaagcc aagaggagga cagttcgcta cacagcaatg 121 catcgagtca ctcagcctct gaagaagctt cgggttcaga ctcaggcagt cagtcggaaa 181 gtgagcaggg aagtgatcca ggaagtggac atggcagcga gtcgaacagc agctctgaat 241 cttctgagag tcagtcggaa tctgagagcg aatcagcagg ttccaaatcc cagccagtcc 301 tcccagaagc caaagagaag ccagcctcta agaaggaacg gatagctgat gtgaagaaga 361 tgtgggaaga atatcctgat gtttatgggg tcaggcggtc aaaccgaagc agacaagaac 421 catcgcgatt taatattaag gaagaggcaa gtagcgggtc tgagagtggg agcccaaaaa 481 gaagaggcca gaggcagctg aaaaaacaag aaaaatggaa acaggaaccc tcagaagatg 541 aacaggaaca aggcaccagt gcagagagtg agccagaaca aaaaaaagta aaagccagaa 601 gacctgtccc cagaagaaca gtgcccaaac ctcgtgttaa aaagcagccg aagactcagc 661 gtggaaagag aaaaaagcaa gattcttctg atgaggatga tgatgatgac gaagctccca 721 aaaggcagac tcgtcgaaga gcggctaaaa acgttagtta caaagaagat gatgactttg 781 agactgactc agatgatctc attgaaatga ctggagaagg agttgatgaa cagcaagata 841 atagtgaaac tattgaaaag gtcttagatt caagactggg aaagaaagga gccactggag 901 catctactac tgtatatgcg attgaagcta atggcgaccc tagtggtgac tttgacactg 961 aaaaggatga aggtgaaatc cagtacctca tcaagtggaa gggttggtct tacatccaca 1021 gcacatggga gagtgaagaa tccttacagc aacagaaagt gaagggccta aaaaaactag 1081 agaacttcaa gaaaaaagag gacgaaatca aacaatggtt agggaaagtt tctcctgaag 1141 atgtagaata tttcaattgc caacaggagc tggcttcaga gttgaataaa cagtatcaga 1201 tagtagaaag agtaATAGCT GTGAAGACAA GTAAATCTAC ATTGGGTCAA ACAGATTTTC 1261 CAGCTCATAG TCGGAAGCCG GCACCCTCAA ATGAGCCCGA ATATCTATGT AAATGGATGG 1321 GACTCCCCTA TTCAGAGTGT AGCTGGGAAG ATGAAGCCCT CATTGGAAAG AAATTCCAGA 1381 ATTGCATTGA CAGCTTCCAC AGTAGGAACA ACTCAAAAAC CATCCCAACA AGAGAATGCA 1441 AGGCCCTGAA GCAGAGACCA CGATTTGTAG CTTTAAAGAA ACAACCTGCA TATTTAGGAG 1501 GGGAGAATCT GGAACTTCGA GATTATCAGC TAGAAGGTCT AAACTGGCTA GCTCATTCCT 1561 GGTGCAAGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1621 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1681 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGAGAGA ACCATGAACG TTGCGATTAC 1741 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt