Construct: ORF TRCN0000466639
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014451.1_s317c1
- Derived from:
- ccsbBroadEn_02613
- DNA Barcode:
- ATGACTTGCGCGCAGGGGCCATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STMN2 (11075)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466639
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11075 | STMN2 | stathmin 2 | NM_007029.4 | 100% | 100% | |
| 2 | human | 11075 | STMN2 | stathmin 2 | XM_005251142.2 | 97.2% | 97.2% | 0_1insATGGCTAAAACAGCA |
| 3 | human | 11075 | STMN2 | stathmin 2 | NM_001199214.2 | 86.4% | 85.9% | (many diffs) |
| 4 | mouse | 20257 | Stmn2 | stathmin-like 2 | NM_025285.2 | 92.7% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 603
- ORF length:
- 537
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc taaaacagca atggcctaca aggaaaaaat gaaggagctg tccatgctgt 121 cactgatctg ctcttgcttt tacccggaac ctcgcaacat caacatctat acttacgatg 181 atatggaagt gaagcaaatc aacaaacgtg cctctggcca ggcttttgag ctgatcttga 241 agccaccatc tcctatctca gaagccccac gaactttagc ttctccaaag aagaaagacc 301 tgtcccTGGA GGAGATCCAG AAGAAACTGG AGGCTGCAGA GGAAAGAAGA AAGTCTCAGG 361 AGGCCCAGGT GCTGAAACAA TTGGCAGAGA AGAGGGAACA CGAGCGAGAA GTCCTTCAGA 421 AGGCTTTGGA GGAGAACAAC AACTTCAGCA AGATGGCGGA GGAAAAGCTG ATCCTGAAAA 481 TGGAACAAAT TAAGGAAAAC CGTGAGGCTA ATCTAGCTGC TATTATTGAA CGTCTGCAGG 541 AAAAGGAGAG GCATGCTGCG GAGGTGCGCA GGAACAAGGA ACTCCAGGTT GAACTGTCTG 601 GCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 661 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 721 GGCTTTATAT ATCTTGTGGA AAGGACGAAT GACTTGCGCG CAGGGGCCAT TCACGCGTTA 781 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt