Construct: ORF TRCN0000466640
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016750.1_s317c1
- Derived from:
- ccsbBroadEn_12857
- DNA Barcode:
- CTATATCTGCGACTTATCGGCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF607 (84775)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466640
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5245 | PHB | prohibitin | NM_001281497.1 | 85.2% | 79.8% | (many diffs) |
| 2 | human | 5245 | PHB | prohibitin | NM_001281496.1 | 53% | 49.6% | (many diffs) |
| 3 | human | 5245 | PHB | prohibitin | NM_001281715.1 | 53% | 49.6% | (many diffs) |
| 4 | human | 5245 | PHB | prohibitin | NM_002634.4 | 53% | 49.6% | (many diffs) |
| 5 | human | 5245 | PHB | prohibitin | XM_017024763.1 | 53% | 49.6% | (many diffs) |
| 6 | mouse | 237880 | 1700071K01Rik | RIKEN cDNA 1700071K01 gene | NM_001033765.2 | 49.3% | 47.6% | (many diffs) |
| 7 | mouse | 18673 | Phb | prohibitin | NM_008831.4 | 49% | 49.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 534
- ORF length:
- 468
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgccaaagtg tttgagtcca tcggcaagtt tggcctggcc ttagctgttg 121 caggaggcat ggtgacctct gccttatgta atgtggatgc tgggcacaga gctgccatct 181 ttgaccaatt ccgtggagta cagaacattg tggtaggGGA AGGGACTCAC TTTCTCATCC 241 CATGTGTACA AAAACCAATT ATCTTTGACT GCTGTTCTCA ACCACGTAGT GCGCCAGTCA 301 TCACTGGTAG CAAAGATTTA CAGAATGTCA ACATCACACT GTGCATCCTC TTCCGGCCCA 361 TCACTAGCCA GCTTCCTCGC ATCTTCACCA GCATTGGAGA GGACTACGAT GAGTGTGTGC 421 TGCCGTTCAT TACCACGGAG ATCCTCAAGT CACTGGTGGC TCGCTTTGAT GCTGGAGAAC 481 TAATCACCCA GAGGGAGCTG GTCTCCAGCC AGGTGAGCAA CAACCTTATG GAGTGCCCAA 541 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 601 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 661 TATCTTGTGG AAAGGACGAC TATATCTGCG ACTTATCGGC CCCACGCGTT AAGTCgacaa 721 tcaacctctg gattacaaaa tttgtgaaag att