Construct: ORF TRCN0000466649
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015942.1_s317c1
- Derived from:
- ccsbBroadEn_05433
- DNA Barcode:
- AACTCACAACGTTCTGCATCCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRTAP19-7 (337974)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466649
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 337974 | KRTAP19-7 | keratin associated protein ... | NM_181614.2 | 100% | 100% | |
| 2 | human | 337972 | KRTAP19-5 | keratin associated protein ... | NM_181611.3 | 77.5% | 68% | (many diffs) |
| 3 | human | 337970 | KRTAP19-3 | keratin associated protein ... | NM_181609.4 | 70.2% | 65.4% | (many diffs) |
| 4 | human | 337971 | KRTAP19-4 | keratin associated protein ... | NM_181610.3 | 64.1% | 57.4% | (many diffs) |
| 5 | human | 337882 | KRTAP19-1 | keratin associated protein ... | NM_181607.3 | 60.3% | 54.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 186
- ORF end:
- 375
- ORF length:
- 189
- Sequence:
-
1 GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT 61 TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT 121 TCCATTTCAG GTGTCGTGAG GCTAGCATCG ATTGATCAAC AAGTTTGTAC AAAAAAGTTG 181 GCACCATGAG CTACTCCGGC AGCTACTATG GAGGCCTAGG CTACGGCTGT GGAGGATTCG 241 GTGGCCTGGG CTATGGCTAT AGCTGTGGAT GTGGCAGCTT CCGCAGACTG GGCTATGGCT 301 GTGGCTATGG AGGCTACAGA TACAGCTGCT GCCACCCATC ATGCTATGGG GGATACTGGT 361 CTTCTGGATT CTATTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 421 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 481 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACTCACAAC GTTCTGCATC 541 CTTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt