Construct: ORF TRCN0000466655
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002661.1_s317c1
- Derived from:
- ccsbBroadEn_00417
- DNA Barcode:
- CCATGGGAATCCTCTGTAAGTCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DAB2 (1601)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466655
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1601 | DAB2 | DAB adaptor protein 2 | NM_001343.4 | 100% | 100% | |
| 2 | human | 1601 | DAB2 | DAB adaptor protein 2 | NM_001244871.1 | 97.2% | 97.2% | 624_625ins63 |
| 3 | mouse | 13132 | Dab2 | disabled 2, mitogen-respons... | NM_023118.5 | 84.7% | 83.3% | (many diffs) |
| 4 | mouse | 13132 | Dab2 | disabled 2, mitogen-respons... | XM_011245324.1 | 84.7% | 83.3% | (many diffs) |
| 5 | mouse | 13132 | Dab2 | disabled 2, mitogen-respons... | XM_017316424.1 | 84.7% | 83.3% | (many diffs) |
| 6 | mouse | 13132 | Dab2 | disabled 2, mitogen-respons... | NM_001310446.1 | 82.1% | 80.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2376
- ORF length:
- 2310
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc taacgaagta gaaacaagtg caaccaatgg tcagcccgac caacaggccg 121 caccaaaagc accctcaaag aaggaaaaaa agaaaggccc tgaaaagaca gatgaatatc 181 tcttagcaag gttcaaaggc gatggtgtaa aatataaggc caagctgatt ggcattgatg 241 atgtgccaga tgcaagaggg gataaaatga gccaagactc tatgatgaaa ctaaagggaa 301 tggcggcagc tggtcggtct cagggacaac acaaacaaag gatctgggtc aacatttccc 361 tttctgggat aaaaataatt gatgagaaaa ctggggtaat agagcatgaa catccagtaa 421 ataagatttc tttcattgcc cgtgatgtga cagacaaccg ggcatttggt tacgtgtgtg 481 gaggagaagg ccagcatcag ttttttgcca taaaaaccgg gcaacaggct gaaccattag 541 ttgttgatct taaagacctt tttcaagtta tctataatgt aaagaaaaag gaagaagaaa 601 agaaaaagat agaggaagcc agcaaagcag ttgagaatgg gagtgaggcc ctaatgattc 661 tagatgacca aactaacaaa ctgaaatcgg gtgttgacca gatggatttg tttggggaca 721 tgtctacacc tcctgaccta aatagtccaa cagaaagcaa agatatcctg ttagtggatc 781 taaactctga aatcgacacc aatcagaatt ctttaagaga aaatccattc ttaacaaacg 841 gcatcacctc ctgttctctt cctcgaccaa cgcctcaggc atccttcttg cctgaaaatg 901 ccttttctgc caatctcaac ttctttccca cccctaatcc tgatcctttc cgtgacgatc 961 ctttcacaca gccagaccaa tcgacacctt cttcgtttga ttctctcaaa tctccagatc 1021 agaagaaaga gaattcgagt agctcgtcta ctccgctgag taatgggccc ctgaatggtg 1081 atgttgacta ctttggtcag caatttgacc agatctctaa ccggactggc aaacaggaag 1141 ctcaggcagg cccatggccc ttttcaagtt cgcaaaccca gccagcagtg agaactcaaa 1201 atggggtatc tgaaagagaa cagaacggct tctctgtcaa atcctccccg aacccttttg 1261 tgggaagccc tcccaaagga ctgtccatac agaatggcgt aaagcaggac ttggaaagct 1321 ctgtccagtc ctcaccacat gactccatag ccattatccc acctccacaa agtaccaaac 1381 caggaagagg cagaaggact gctaagtctt cagccaatga cttgcttgca tcagacatct 1441 ttgctcctcc cgtctcagaa ccttcaggcc aggcgtcacc cacaggacaa cctacagccc 1501 tgcagcccaa ccctctggat ctcttcaaaa caagtgctcc tgccccagtg gggcccctgg 1561 tgggtctagg tggtgtaact gtcacactcc ctcaggcagg accatggaac acagcatctt 1621 tggtcttcaa tcagtcccct tcaatggctc cgggagccat gatgggtggt caaccttcag 1681 gttttagtca gcccgtcatt tttggtacaa gtccagctgt ttcaggttgg aaccagcctt 1741 caccctttgc agcctcaact ccccctccag tgcctgttgt ctggggccct tctgcatctg 1801 tggcacccaa tgcttggtca acaacaagcc ctttggggaa tccttttcag agcaatattt 1861 ttccagctcc tgctgtgtcc actcagcccc catccatgca ctcctctctc ctggtcactc 1921 ctcctcagcc acctcccaga gctggccctc ccaaggacat ctccagtgat gccttcactg 1981 ccTTAGACCC ACTTGGGGAT AAAGAGATCA AGGATGTGAA AGAAATGTTT AAGGATTTCC 2041 AACTGCGGCA GCCACCTGCT GTGCCCGCGC GGAAGGGAGA GCAGACTTCT TCTGGGACTT 2101 TGAGTGCCTT TGCCAGTTAT TTCAACAGCA AGGTTGGCAT TCCTCAGGAG AATGCAGACC 2161 ATGATGACTT TGATGCTAAT CAACTATTGA ACAAGATCAA TGAACCACCA AAGCCAGCTC 2221 CCAGACAAGT TTCCCTGCCA GTTACCAAAT CTACTGACAA TGCATTTGAG AACCCTTTCT 2281 TTAAAGATTC TTTTGGTTCA TCACAAGCCT CTGTGGCTTC TTCTCAACCT GTATCTTCTG 2341 AGATGTATAG GGATCCATTT GGAAATCCTT TTGCCTACCC AACTTTCTTG TACAAAGTGG 2401 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2461 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2521 ACCATGGGAA TCCTCTGTAA GTCAGACGCG TTAAGTCgac aatcaacctc tggattacaa 2581 aatttgtgaa agatt