Construct: ORF TRCN0000466687
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015050.1_s317c1
- Derived from:
- ccsbBroadEn_02719
- DNA Barcode:
- CAACGATCGAGTTGTAACGAAGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SWAP70 (23075)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466687
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23075 | SWAP70 | switching B cell complex su... | NM_015055.4 | 100% | 100% | |
| 2 | human | 23075 | SWAP70 | switching B cell complex su... | NM_001297714.2 | 90% | 90% | 238_239ins174 |
| 3 | human | 23075 | SWAP70 | switching B cell complex su... | XM_005252830.4 | 74.5% | 74.5% | 0_1ins447 |
| 4 | mouse | 20947 | Swap70 | SWA-70 protein | NM_009302.3 | 89.5% | 95% | (many diffs) |
| 5 | mouse | 20947 | Swap70 | SWA-70 protein | XM_017322086.1 | 48.9% | 52.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1821
- ORF length:
- 1755
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gagcttgaag gaggagctgc tcaaagccat ctggcacgcc ttcaccgcac 121 tcgaccagga ccacagcggc aaggtctcca agtcccagct caaggtcctt tcccataacc 181 tgtgcacggt gctgaaggtt cctcatgacc cagttgccct tgaagagcac ttcagggatg 241 atgatgaggg tccagtgtcc aaccagggct acatgcctta tttaaacagg ttcattttgg 301 aaaaggtcca agacaacttt gacaagattg aattcaatag gatgtgttgg accctctgtg 361 tcaaaaaaaa cctcacaaag aatcccctgc tcattacaga agaagatgca tttaaaatat 421 gggttatttt caacttttta tctgaggaca agtatccatt aattattgtg tcagaagaga 481 ttgaatacct gcttaagaag cttacagaag ctatgggagg aggttggcag caagaacaat 541 ttgaacatta taaaatcaac tttgatgaca gtaaaaatgg cctttctgca tgggaactta 601 ttgagcttat tggaaatgga cagtttagca aaggcatgga ccggcagact gtgtctatgg 661 caattaatga agtctttaat gaacttatat tagatgtgtt aaagcagggt tacatgatga 721 aaaagggcca cagacggaaa aactggactg aaagatggtt tgtactaaaa cccaacataa 781 tttcttacta tgtgagtgag gatctgaagg ataagaaagg agacattctc ttggatgaaa 841 attgctgtgt agagtccttg cctgacaaag atggaaagaa atgccttttt ctcgtaaaat 901 gttttgataa gacttttgaa atcagtgctt cagataagaa gaagaaacag gagtggattc 961 aagccattca ttctactatt catctgttga agctgggcag ccctccacca cacaaagaag 1021 cccgccagcg tcggaaagaa ctccggaaga agcagctggc tgaacaagag gaactggagc 1081 gacaaatgaa ggaactccag gccgccaacg aaagcaagca gcaggagctg gaggccgtgc 1141 ggaagaaact ggaggaagca gcatctcgtg cagcagaaga ggaaaagaaa cgccttcaga 1201 ctcaagtgga acttcaggcc aggttcagca cagagctgga aagagagaag cttatcagac 1261 agcagatgga agaacaggtt gctcaaaagt cctctgaact ggaacagtat ttacagcgag 1321 tacgggagct ggaagacatg tacctaaagc tgcaggaggc tcttgaagat gagagacagg 1381 cccggcaaga tgaagagaca gtgcggaagc ttcaggccag gttgttggag gaagagtctt 1441 ccaagagggc tgaactagaa aagtggcact tggagcagca gcaggccatt cagacaaccg 1501 aggcggagaa gcaggagttg gagaaTCAGC GTGTCCTGAA GGAACAGGCC CTGCAGGAGG 1561 CCATGGAGCA GCTGGAGCAG CTTGAGTTAG AACGGAAGCA AGCACTTGAG CAGTACGAGG 1621 AAGTTAAAAA GAAGCTGGAG ATGGCAACTA ATAAGACCAA GAGCTGGAAG GACAAAGTGG 1681 CCCATCATGA AGGATTAATT CGACTGATAG AACCAGGTTC AAAGAACCCT CACCTGATCA 1741 CTAACTGGGG ACCTGCAGCT TTCACTGAGG CAGAACTTGA AGAGAGAGAG AAGAACTGGA 1801 AAGAGAAAAA GACCACGGAG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1861 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1921 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACAAC GATCGAGTTG 1981 TAACGAAGGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt