Construct: ORF TRCN0000466691

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016325.1_s317c1
Derived from:
ccsbBroadEn_10843
DNA Barcode:
TCCAACGCAGCACACTCACTGTGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GCSH (2653)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466691
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2653 GCSH glycine cleavage system pro... NM_004483.5 56.2% 56% 62C>T;294_519delinsC
2 human 2653 GCSH glycine cleavage system pro... XM_017023136.2 54.3% 54.1% 62C>T;294_537delinsC
3 human 729080 LOC729080 glycine cleavage system pro... NR_033244.1 25.9% (many diffs)
4 human 641746 LOC641746 glycine cleavage system pro... NR_033245.1 22.2% (many diffs)
5 human 2653 GCSH glycine cleavage system pro... NR_033249.2 18.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
360
ORF length:
294
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gctgcgagtg gtgcggagcg tgcgggccct gctctgcacc cTGCGCGCGG
121TCCCGTTACC CGCCGCGCCC TGCCCGCCGA GGCCCTGGCA GCTGGGGGTG GGCGCCGTCC
181GTACGCTGCG CACTGGACCC GCTCTGCTCT CGGTGCGTAA ATTCACAGAG AAACACGAAT
241GGGTAACAAC AGAAAATGGC ATTGGAACAG TGGGAATCAG CAATTTTGCA CAGGAAGCGT
301TGGGAGATGT TGTTTATTGT AGTCTCCCTG AAGTTGGGAC AAAATTGAAC AAACAAGACT
361ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
421GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
481TTTATATATC TTGTGGAAAG GACGATCCAA CGCAGCACAC TCACTGTGGA CGCGTTAAGT
541Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)