Construct: ORF TRCN0000466705
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017327.1_s317c1
- Derived from:
- ccsbBroadEn_13471
- DNA Barcode:
- GCCCTGCAGACGTGCTATTAGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF144B (255488)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466705
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 255488 | RNF144B | ring finger protein 144B | NM_182757.3 | 99.4% | 99.6% | 669C>T;819A>C;823_825delTTG |
| 2 | human | 255488 | RNF144B | ring finger protein 144B | XM_005248986.3 | 87.8% | 88.1% | (many diffs) |
| 3 | mouse | 218215 | Rnf144b | ring finger protein 144B | NM_001170643.1 | 84.1% | 87.7% | (many diffs) |
| 4 | mouse | 218215 | Rnf144b | ring finger protein 144B | NM_146042.4 | 84.1% | 87.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 972
- ORF length:
- 906
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctcagctggt aggctccact atctcgccat gactgctgaa aatcccactc 121 ctggagacct ggctccggcc cccctcatca cttgcaaact ctgcctgtgt gagcagtctc 181 tggacaagat gaccacactc caggaatgcc agtgcatctt ttgcacagct tgcctgaaac 241 agtacatgca gctggcaatc cgagaaggat gtgggtctcc catcacttgc cctgacatgg 301 tgtgcctaaa ccacgggacc ctgcaggaag ctgagattgc ctgtttggta cctgtggacc 361 agtttcaact ttatcagagg ttaaaatttg aaagagaagt tcatctggac ccctaccgaa 421 catggtgtcc tgttgcagac tgtcagacag tgtgccctgt tgcctcgagt gacccaggac 481 agcctgtgct ggtggaatgc ccttcttgcc acctgaaatt ctgctcgtgt tgcaaggatg 541 cttggcatgc agaggtctcc tgtagagaca gtcagcctat tgtcctgcca acagagcacc 601 gagccctctt tgggacagat gcagaagccc cCATTAAGCA GTGCCCAGTT TGCCGGGTTT 661 ATATCGAACG CAATGAAGGC TGCGCTCAGA TGATGTGCAA AAACTGCAAG CATACATTTT 721 GCTGGTACTG CCTTCAGAAC TTGGATAATG ACATTTTCCT CAGACATTAT GACAAAGGGC 781 CATGCAGGAA TAAACTTGGC CACTCAAGAG CATCAGTGAT GTGGAACCGA ACACAGGTGG 841 TGGGGATTCT CGTAGGCTTG GGCATCATTG CCTTGGTTAC TTCCCCCTTA CTCCTGGCCT 901 CCCCATGTAT AATCTGTTGT GTCTGCAAGT CCTGTCGGGG CAAGAAGAAA AAGCACGACC 961 CATCCACAAC CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1021 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1081 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCC CTGCAGACGT GCTATTAGCG 1141 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t