Construct: ORF TRCN0000466726
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014638.1_s317c1
- Derived from:
- ccsbBroadEn_07419
- DNA Barcode:
- TAACCCTGGTTTCTATGGAAATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOMER3 (9454)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466726
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9454 | HOMER3 | homer scaffold protein 3 | NM_001145722.2 | 99.8% | 99.7% | 271C>T;1026C>G |
| 2 | human | 9454 | HOMER3 | homer scaffold protein 3 | NM_004838.4 | 99.8% | 99.7% | 271C>T;1026C>G |
| 3 | human | 9454 | HOMER3 | homer scaffold protein 3 | XM_006722943.1 | 99.8% | 99.7% | 271C>T;1026C>G |
| 4 | human | 9454 | HOMER3 | homer scaffold protein 3 | XM_006722944.2 | 99.8% | 99.7% | 271C>T;1026C>G |
| 5 | human | 9454 | HOMER3 | homer scaffold protein 3 | NM_001145721.1 | 98.9% | 98.8% | 271C>T;892_893insAGGACCTGG;1017C>G |
| 6 | human | 9454 | HOMER3 | homer scaffold protein 3 | NM_001145724.1 | 89.8% | 89.7% | 271C>T;300_301ins108;918C>G |
| 7 | human | 9454 | HOMER3 | homer scaffold protein 3 | NR_027297.1 | 54.2% | (many diffs) | |
| 8 | mouse | 26558 | Homer3 | homer scaffolding protein 3 | NM_001146153.1 | 82.2% | 89.4% | (many diffs) |
| 9 | mouse | 26558 | Homer3 | homer scaffolding protein 3 | NM_011984.2 | 81.5% | 88.6% | (many diffs) |
| 10 | mouse | 26558 | Homer3 | homer scaffolding protein 3 | XM_006509679.1 | 80.7% | 87.7% | (many diffs) |
| 11 | mouse | 26558 | Homer3 | homer scaffolding protein 3 | XM_006509680.3 | 80.7% | 87.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1149
- ORF length:
- 1083
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cacagccagg gagcagccaa tcttcagcac acgggcgcac gtgttccaaa 121 ttgacccagc caccaagcga aactggatcc cagcgggcaa gcacgcactc actgtctcct 181 atttctacga tgccacccgc aatgtgtacc gcatcatcag catcggaggc gccaaggcca 241 tcatcaacag cactgtcact cccaacatga ccttcaccaa aacttcccag aagttcgggc 301 agtgggccga cagtcgcgcc aacacagtct acggcttggg ctttgcctct gaacagcatc 361 tgacacagtt tgccgagaag ttccaggaag tgaaggaagc agccaggctg gccagggaga 421 aatctcagga tggcggggag ctcaccagtc cagccctggg gctcgcctcc caccaggtgc 481 ccccgagccc tctcgtcagt gccaacggcc ccggcgagga aaaactgttc cgcagccaga 541 gcgctgatgc ccccggcccc acagagcgcg agcggctaaa gaagatgttg tctgagggct 601 ccgtgggcga ggtacagtgg gaggccgagt ttttcgcact gcaggacagc aacaacaagc 661 tggcaggcgc cctgcgagag gccaacgccg ccgcagccca gtggaggcag cagctggagg 721 ctcagcgtgc agaggccgag cggctgcggc agcgggtggc tgagctggag gcTCAGGCAG 781 CTTCAGAGGT GACCCCCACC GGTGAGAAGG AGGGGCTGGG CCAGGGCCAG TCGCTGGAAC 841 AGCTGGAAGC TCTGGTGCAA ACCAAGGACC AGGAGATTCA GACCCTGAAG AGTCAGACTG 901 GGGGGCCCCG CGAGGCCCTG GAGGCTGCCG AGCGTGAGGA GACTCAGCAG AAGGTGCAGG 961 ACCTGGAGAC CCGCAATGCG GAGTTGGAGC ACCAGCTGCG GGCGATGGAG CGCAGCCTGG 1021 AGGAGGCACG GGCAGAGCGG GAGCGGGCGC GGGCTGAGGT GGGCCGGGCA GCGCAGCTGC 1081 TGGACGTCAG GCTGTTTGAG CTGAGTGAGC TGCGTGAGGG CCTGGCCCGC CTGGCTGAGG 1141 CTGCGCCCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1261 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATAACCC TGGTTTCTAT GGAAATCCAC 1321 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt