Construct: ORF TRCN0000466733
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005637.1_s317c1
- Derived from:
- ccsbBroadEn_01950
- DNA Barcode:
- ATCGATACCGAATTCTCCGGACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CGGBP1 (8545)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466733
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8545 | CGGBP1 | CGG triplet repeat binding ... | NM_001008390.2 | 100% | 100% | |
| 2 | human | 8545 | CGGBP1 | CGG triplet repeat binding ... | NM_001195308.1 | 100% | 100% | |
| 3 | human | 8545 | CGGBP1 | CGG triplet repeat binding ... | NM_003663.3 | 100% | 100% | |
| 4 | human | 8545 | CGGBP1 | CGG triplet repeat binding ... | XM_024453797.1 | 100% | 100% | |
| 5 | mouse | 106143 | Cggbp1 | CGG triplet repeat binding ... | NM_178647.2 | 91.6% | 98.2% | (many diffs) |
| 6 | mouse | 106143 | Cggbp1 | CGG triplet repeat binding ... | XM_006521658.2 | 91.6% | 98.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 567
- ORF length:
- 501
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcgatttgta gtaacagcac cacctgctcg aaaccgttct aagactgctt 121 tgtatgtgac tcccctggat cgagtcactg agtttGGAGG TGAGCTGCAT GAAGATGGAG 181 GAAAACTCTT CTGCACTTCT TGCAATGTGG TTCTGAATCA TGTTCGCAAG TCTGCCATTA 241 GTGACCACCT CAAGTCAAAG ACTCATACCA AGAGGAAGGC AGAATTTGAA GAGCAGAATG 301 TGAGAAAGAA GCAGAGGCCC CTAACTGCAT CTCTTCAGTG CAACAGTACT GCGCAAACAG 361 AGAAAGTCAG TGTTATCCAG GACTTTGTGA AAATGTGCCT GGAAGCCAAC ATCCCACTTG 421 AGAAGGCTGA TCACCCAGCA GTCCGTGCTT TCCTATCTCG CCATGTGAAG AATGGAGGCT 481 CCATACCTAA GTCAGACCAG CTACGGAGGG CATATCTTCC TGATGGATAT GAGAATGAGA 541 ATCAACTCCT CAACTCACAA GATTGTTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 601 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 661 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATCGATAC 721 CGAATTCTCC GGACTGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 781 aagatt