Construct: ORF TRCN0000466734
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018522.1_s317c1
- Derived from:
- ccsbBroadEn_10707
- DNA Barcode:
- CGCCCTGATTCCAAAGGCTGCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CACNB1 (782)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466734
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 782 | CACNB1 | calcium voltage-gated chann... | NM_000723.5 | 98.8% | 98.6% | 1_18del;791T>C;1142C>A |
| 2 | human | 782 | CACNB1 | calcium voltage-gated chann... | XM_017025025.1 | 92.7% | 91.3% | (many diffs) |
| 3 | human | 782 | CACNB1 | calcium voltage-gated chann... | XM_005257645.2 | 91.7% | 90.8% | (many diffs) |
| 4 | human | 782 | CACNB1 | calcium voltage-gated chann... | XM_006722072.2 | 85.9% | 83.9% | (many diffs) |
| 5 | human | 782 | CACNB1 | calcium voltage-gated chann... | XM_017025024.1 | 85.4% | 83.8% | (many diffs) |
| 6 | human | 782 | CACNB1 | calcium voltage-gated chann... | NM_199248.3 | 76% | 72% | (many diffs) |
| 7 | human | 782 | CACNB1 | calcium voltage-gated chann... | NM_199247.3 | 70.5% | 66.2% | (many diffs) |
| 8 | human | 782 | CACNB1 | calcium voltage-gated chann... | XM_017025026.1 | 68.8% | 66.6% | (many diffs) |
| 9 | human | 782 | CACNB1 | calcium voltage-gated chann... | XM_024450920.1 | 68.8% | 66.6% | (many diffs) |
| 10 | human | 782 | CACNB1 | calcium voltage-gated chann... | XM_017025027.1 | 62.3% | 62.1% | 0_1ins666;107T>C;458C>A |
| 11 | human | 782 | CACNB1 | calcium voltage-gated chann... | XM_017025028.2 | 62.3% | 62.1% | 0_1ins666;107T>C;458C>A |
| 12 | human | 782 | CACNB1 | calcium voltage-gated chann... | XM_017025029.1 | 62.3% | 62.1% | 0_1ins666;107T>C;458C>A |
| 13 | human | 782 | CACNB1 | calcium voltage-gated chann... | XM_017025030.1 | 58.1% | 57.3% | (many diffs) |
| 14 | human | 782 | CACNB1 | calcium voltage-gated chann... | XM_005257646.3 | 53.7% | 52.4% | (many diffs) |
| 15 | mouse | 12295 | Cacnb1 | calcium channel, voltage-de... | NM_145121.3 | 91.1% | 96.9% | (many diffs) |
| 16 | mouse | 12295 | Cacnb1 | calcium channel, voltage-de... | XM_006532092.2 | 87.4% | 91.3% | (many diffs) |
| 17 | mouse | 12295 | Cacnb1 | calcium channel, voltage-de... | NM_001282977.1 | 84.5% | 89.2% | (many diffs) |
| 18 | mouse | 12295 | Cacnb1 | calcium channel, voltage-de... | XM_006532091.2 | 81.3% | 84.2% | (many diffs) |
| 19 | mouse | 12295 | Cacnb1 | calcium channel, voltage-de... | NM_001159319.2 | 70.6% | 71.5% | (many diffs) |
| 20 | mouse | 12295 | Cacnb1 | calcium channel, voltage-de... | NM_001282978.1 | 69.3% | 70.2% | (many diffs) |
| 21 | mouse | 12295 | Cacnb1 | calcium channel, voltage-de... | NM_031173.4 | 65.5% | 65.7% | (many diffs) |
| 22 | mouse | 12295 | Cacnb1 | calcium channel, voltage-de... | XM_017314236.1 | 62.8% | 61.4% | (many diffs) |
| 23 | mouse | 12295 | Cacnb1 | calcium channel, voltage-de... | NM_001159320.2 | 60.1% | 58.9% | (many diffs) |
| 24 | mouse | 12295 | Cacnb1 | calcium channel, voltage-de... | XM_006532094.2 | 56.8% | 60.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1842
- ORF length:
- 1776
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ccggggccct tacccaccct cccaggagat ccccatggag gtcttcgacc 121 ccagcccgca gggcaaatac agcaagagga aagggcgatt caaacggtca gatgggagca 181 cgtcctcgga taccacatcc aacagctttg tccgccaggg ctcagcggag tcctacacca 241 gccgtccatc agactctgat gtatctctgg aggaggaccg ggaagcctta aggaaggaag 301 cagagcgcca ggcattagcg cagctcgaga aggccaagac caagccagtg gcatttgctg 361 tgcggacaaa tgttggctac aatccgtctc caggggatga ggtgcctgtg cagggagtgg 421 ccatcacctt cgagcccaaa gacttcctgc acatcaagga gaaatacaat aatgactggt 481 ggatcgggcg gctggtgaag gagggctgtg aggttggctt cattcccagc cccgtcaaac 541 tggacagcct tcgcctgctg caggaacaga agctgcgcca gaaccgcctc ggctccagca 601 aatcaggcga taactccagt tccagtctgg gagatgtggt gactggcacc cgccgcccca 661 caccccctgc cagtgccaaa cagaagcaga agtcgacaga gcatgtgccc ccctatgacg 721 tggtgccttc catgaggccc atcatcctgg tgggaccgtc gctcaagggc tacgaggtta 781 cagacatgat gcagaaagct ttatttgact tcttgaagca tcggtttgat ggcaggacct 841 ccatcactcg tgtgacggca gatatttccc tggctaagcg ctcagttctc aacaacccca 901 gcaaacacat catcattgag cgctccaaca cacgctccag cctggctgag gtgcagagtg 961 aaatcgagcg aatcttcgag ctggcccgga cccttcagtt ggtcgctctg gatgctgaca 1021 ccatcaatca cccagcccag ctgtccaaga cctcgctggc ccccatcatt gtttacatca 1081 agatcacctc tcccaaggta cttcaaaggc tcatcaagtc ccgaggaaag tctcagtcca 1141 aacacctcaa tgtccaaata gcggcctcgg aaaagctggc acagtgccac cctgaaatgt 1201 ttgacatcat cctggatgag aaccaattgg aggatgcctg cgagcatctg gcggagtact 1261 tggaagccta ttggaaggcc acacacccgc ccagcagcac gccacccaat ccgctgctga 1321 accgcaccat ggctaccgca gccctggctg ccagccctgc ccctgtctcc aacctccagg 1381 gaccctacct tgcttccggg gaccagccac tggaacgggc caccggggag cacgccagca 1441 tgcacgagta cccaggGGAG CTGGGCCAGC CCCCAGGCCT TTACCCCAGC AGCCACCCAC 1501 CAGGCCGGGC AGGCACGCTA CGGGCACTGT CCCGCCAAGA CACTTTTGAT GCCGACACCC 1561 CCGGCAGCCG AAACTCTGCC TACACGGAGC TGGGAGACTC ATGTGTGGAC ATGGAGACTG 1621 ACCCCTCAGA GGGGCCAGGG CTTGGAGACC CTGCAGGGGG CGGCACGCCC CCAGCCCGAC 1681 AGGGATCCTG GGAGGACGAG GAAGAAGACT ATGAGGAAGA GCTGACCGAC AACCGGAACC 1741 GGGGCCGGAA TAAGGCCCGC TACTGCGCTG AGGGTGGGGG TCCAGTTTTG GGGCGCAACA 1801 AGAATGAGCT GGAGGGCTGG GGACGAGGCG TCTACATTCG CTGCCCAACT TTCTTGTACA 1861 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1921 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1981 AGGACGACGC CCTGATTCCA AAGGCTGCAT GACGCGTTAA GTCgacaatc aacctctgga 2041 ttacaaaatt tgtgaaagat t