Construct: ORF TRCN0000466742
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011414.1_s317c1
- Derived from:
- ccsbBroadEn_16116
- DNA Barcode:
- TCGATAGTAACTATTTTGCCTAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNST (163882)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466742
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 163882 | CNST | consortin, connexin sorting... | NM_001139459.1 | 41% | 38% | (many diffs) |
| 2 | human | 163882 | CNST | consortin, connexin sorting... | NM_152609.3 | 34.9% | 32.3% | (many diffs) |
| 3 | human | 163882 | CNST | consortin, connexin sorting... | XM_005273081.3 | 34.9% | 32.3% | (many diffs) |
| 4 | human | 163882 | CNST | consortin, connexin sorting... | XM_011544110.3 | 34.9% | 32.3% | (many diffs) |
| 5 | human | 163882 | CNST | consortin, connexin sorting... | XM_011544111.1 | 34.9% | 32.3% | (many diffs) |
| 6 | human | 163882 | CNST | consortin, connexin sorting... | XM_017000487.1 | 17.9% | 15.1% | (many diffs) |
| 7 | human | 163882 | CNST | consortin, connexin sorting... | XM_011544112.1 | 16.2% | 13.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 855
- ORF length:
- 789
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgacagcgat actcctacat attatctgca aatagaacca caagatggat 121 gtcatcctgg tgacagcgtg gaaaggagtg tgacctgtct gccttctgca tcagatgaaa 181 atgaaaatca gcttgacggg gacgggcatg agcatctgac cagcagtgac agtgcgatgg 241 gaaagcccca agtgtctgag caggacagtc tcaataataa tgaaagctgc acattgagct 301 gcgaggtggc tgcaggtgag aacttgcaaa acaccctttg tgaagcctcc agagatgaac 361 aggccttctt gggaaaggac aaaaaaattc ctggaaaaag aagtccaaga agcaaaaaag 421 ggactgctaa gaagatacca ccaggacttt tttcaggaga tattgcacct ttaatgcaag 481 aaaaagtact aagcgcagtc acatatgctg ttgatgatga agaagctgct gaagtaaatg 541 ctaatgagca gccagaggcg ccaaagcttg ttctgcagtc tctgttttca cttatacgag 601 gtgaagttga gcagttGGAT TCAAGAGCAC TTCCCCTTTG CCTTCATCAG ATAGCAGAAT 661 CCTATTTCCA GGAGGAGGAC TATGAGAAAG CAATGAAATT CATTCAGCTA GAACGATTGT 721 ATCATGAGCA ATTGCTCGCA AATCTTTCTG CCATTCAAGA ACAGTGGGGT AAGTACAGAC 781 CAACATGGAA TGTGGAATTA GCAGTAATCC AACAAGTCAC TATCTACAAA ATTAACATAT 841 TTGACCTGGT TTTATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 901 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 961 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCGATAGTAA CTATTTTGCC 1021 TAATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt