Construct: ORF TRCN0000466743
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012682.2_s317c1
- Derived from:
- ccsbBroadEn_09823
- DNA Barcode:
- TTGCGTGTGATGTTCCCACTTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK32A (202374)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466743
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 202374 | STK32A | serine/threonine kinase 32A | NM_145001.4 | 99.7% | 100% | 9G>C |
| 2 | human | 202374 | STK32A | serine/threonine kinase 32A | XM_011537579.2 | 72.5% | 68.1% | (many diffs) |
| 3 | human | 202374 | STK32A | serine/threonine kinase 32A | XM_017009213.1 | 52.3% | 49.5% | (many diffs) |
| 4 | human | 202374 | STK32A | serine/threonine kinase 32A | NM_001112724.2 | 41.2% | 39.2% | (many diffs) |
| 5 | human | 202374 | STK32A | serine/threonine kinase 32A | XM_011537577.2 | 40.9% | 38.9% | (many diffs) |
| 6 | human | 202374 | STK32A | serine/threonine kinase 32A | NM_001287740.2 | 40.1% | 38.1% | (many diffs) |
| 7 | human | 202374 | STK32A | serine/threonine kinase 32A | XM_011537576.1 | 37.6% | 34.5% | (many diffs) |
| 8 | human | 202374 | STK32A | serine/threonine kinase 32A | XR_001742038.1 | 14.9% | (many diffs) | |
| 9 | human | 202374 | STK32A | serine/threonine kinase 32A | XR_001742037.1 | 6.8% | (many diffs) | |
| 10 | mouse | 269019 | Stk32a | serine/threonine kinase 32A | NM_178749.3 | 38.5% | 37.2% | (many diffs) |
| 11 | mouse | 64293 | Stk32b | serine/threonine kinase 32B | NM_022416.2 | 32.4% | 33.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 564
- ORF length:
- 498
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg agccaacact tcaagaaaac caccagtgtt tgatgaaaat gaagatgtca 121 actttgacca ctttgaaatt ttgcgagcca ttgggaaagg cagttttggg aaggtctgca 181 ttgtacagaa gaatgatacc aagaagatgt acgcaatgaa gtacatgaat aaacaaaagt 241 gcgtggagcg caatgaagtg agaaatgtcT TCAAGGAACT CCAGATCATG CAGGGTCTGG 301 AGCACCCTTT CCTGGTTAAT TTGTGGTATT CCTTCCAAGA TGAGGAAGAC ATGTTCATGG 361 TGGTGGACCT CCTGCTGGGT GGAGACCTGC GTTATCACCT GCAACAGAAC GTCCACTTCA 421 AGGAAGAAAC AGTGAAGCTC TTCATCTGTG AGCTGGTCAT GGCCCTGGAC TACCTGCAGA 481 ACCAGCGCAT CATTCACAGG GATATGAAGC CTGACAATAT TTTACTTGAC GAACATGATA 541 CCTGGCTCTC CTACAAGTCC CACTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 601 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 661 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TGCGTGTGAT 721 GTTCCCACTT ATCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 781 att