Construct: ORF TRCN0000466753
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002442.1_s317c1
- Derived from:
- ccsbBroadEn_01126
- DNA Barcode:
- TGTACGGGGTCCCTGCGTGTATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OSM (5008)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466753
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5008 | OSM | oncostatin M | NM_020530.6 | 100% | 100% | |
| 2 | human | 5008 | OSM | oncostatin M | NM_001319108.2 | 91.6% | 91.6% | 0_1ins63 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 822
- ORF length:
- 756
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggtactgctc acacagagga cgctgctcag tctggtcctt gcactcctgt 121 ttccaagcat ggcgagcatg gcggctatag gcagctgctc gaaagagtac cgcgtgctcc 181 ttggccagct ccagaagcag acagatctca tgcaggacac cagcagactc ctggacccct 241 atatacgtat ccaaggcctg gatgttccta aactgagaga gcactgcagg gagcgccccg 301 gggccttccc cagtgaggag accctgaggg ggctgggcag gcggggcttc ctgcagaccc 361 tcaatgccac actgggctgc gtcctgcaca gactggccga cttagagcag cgcctcccca 421 aggcccagga tttGGAGAGG TCTGGGCTGA ACATCGAGGA CTTGGAGAAG CTGCAGATGG 481 CGAGGCCGAA CATCCTCGGG CTCAGGAACA ACATCTACTG CATGGCCCAG CTGCTGGACA 541 ACTCAGACAC GGCTGAGCCC ACGAAGGCTG GCCGGGGGGC CTCTCAGCCG CCCACCCCCA 601 CCCCTGCCTC GGATGCTTTT CAGCGCAAGC TGGAGGGCTG CAGGTTCCTG CATGGCTACC 661 ATCGCTTCAT GCACTCAGTG GGGCGGGTCT TCAGCAAGTG GGGGGAGAGC CCGAACCGGA 721 GCCGGAGACA CAGCCCCCAC CAGGCCCTGA GGAAGGGGGT GCGCAGGACC AGACCCTCCA 781 GGAAAGGCAA GAGACTCATG ACCAGGGGAC AGCTGCCCCG GTACCCAACT TTCTTGTACA 841 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 901 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 961 AGGACGATGT ACGGGGTCCC TGCGTGTATG TACGCGTTAA GTCgacaatc aacctctgga 1021 ttacaaaatt tgtgaaagat t