Construct: ORF TRCN0000466756
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003025.1_s317c1
- Derived from:
- ccsbBroadEn_03248
- DNA Barcode:
- CAAATACATCGGTACACGCTCTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIGP (51227)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466756
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51227 | PIGP | phosphatidylinositol glycan... | NM_001320480.1 | 100% | 100% | |
| 2 | human | 51227 | PIGP | phosphatidylinositol glycan... | NM_153682.2 | 100% | 100% | |
| 3 | human | 51227 | PIGP | phosphatidylinositol glycan... | NM_153681.2 | 84.8% | 84.8% | 1_72del |
| 4 | human | 51227 | PIGP | phosphatidylinositol glycan... | NM_016430.3 | 80.3% | 79.1% | 1_1delAins74;4_5insCTTCA |
| 5 | human | 51227 | PIGP | phosphatidylinositol glycan... | XM_017028365.1 | 80.3% | 79.1% | 1_1delAins74;4_5insCTTCA |
| 6 | mouse | 56176 | Pigp | phosphatidylinositol glycan... | NM_001159616.1 | 85.5% | 90.2% | (many diffs) |
| 7 | mouse | 56176 | Pigp | phosphatidylinositol glycan... | NM_019543.3 | 85.5% | 90.2% | (many diffs) |
| 8 | mouse | 56176 | Pigp | phosphatidylinositol glycan... | NM_001159618.1 | 70.3% | 74.2% | (many diffs) |
| 9 | mouse | 56176 | Pigp | phosphatidylinositol glycan... | NM_001159619.1 | 68.6% | 72.4% | (many diffs) |
| 10 | mouse | 56176 | Pigp | phosphatidylinositol glycan... | NM_001159617.1 | 55.3% | 58.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 468
- ORF length:
- 402
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gGAAAATTCA CCGTCGCCAT TGCCAGAAAG AGCGATTTAT GGCTTTGTTC 121 TTTTCTTAAG CTCCCAATTT GGCTTCATAC TTTACCTCGT GTGGGCCTTT ATTCCTGAAT 181 CTTGGCTAAA CTCTTTAGGT TTAACCTATT GGCCTCAAAA ATATTGGGCA GTTGCATTAC 241 CTGTCTACCT CCTTATTGCT ATAGTAATTG GCTACGTGCT CTTGTTTGGG ATTAACATGA 301 TGAGTACCTC TCCACTCGAC TCCATCCATA CAATCACAGA TAACTATGCA AAAAATCAAC 361 AGCAGAAGAA ATACCAAGAG GAGGCCATTC CAGCCTTAAG AGATATTTCT ATTAGTGAAG 421 TAAACCAAAT GTTCTTTCTT GCAGCCAAAG AACTTTACAC CAAAAACTGC CCAACTTTCT 481 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 541 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 601 GTGGAAAGGA CGACAAATAC ATCGGTACAC GCTCTTGACG CGTTAAGTCg acaatcaacc 661 tctggattac aaaatttgtg aaagatt