Construct: ORF TRCN0000466757
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008354.1_s317c1
- Derived from:
- ccsbBroadEn_12970
- DNA Barcode:
- GGTTTTCACAATCCACCCCTAGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CALML4 (91860)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466757
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91860 | CALML4 | calmodulin like 4 | NM_001286694.1 | 100% | 100% | |
| 2 | human | 91860 | CALML4 | calmodulin like 4 | NM_001031733.2 | 72.7% | 66.4% | (many diffs) |
| 3 | human | 91860 | CALML4 | calmodulin like 4 | NM_001286695.1 | 67.5% | 67.5% | 0_1ins117 |
| 4 | human | 91860 | CALML4 | calmodulin like 4 | NM_033429.2 | 61.2% | 61.2% | 1_228del |
| 5 | mouse | 75600 | Calml4 | calmodulin-like 4 | XM_006511530.1 | 83% | 85.8% | (many diffs) |
| 6 | mouse | 75600 | Calml4 | calmodulin-like 4 | NM_138304.2 | 66.6% | 67.9% | (many diffs) |
| 7 | mouse | 75600 | Calml4 | calmodulin-like 4 | XM_006511529.3 | 44.2% | 45.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 450
- ORF length:
- 360
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGTGGCCAT GAGGTGCCTG GGGGCCAGCC 121 CGACGCCAGG GGAGGTGCAG CGGCACCTGC AGACCCACGG GATAGACGGA AATGGAGAGC 181 TGGATTTCTC CACTTTTCTG ACCATTATGC ACATGCAAAT AAAACAAGAA GACCCAAAGA 241 AAGAAATTCT TCTAGCCATG TTGATGGTGG ACAAGGAGAA GAAAGGTTAC GTCATGGCGT 301 CCGACCTGCG GTCAAAACTC ACGAGTCTGG GGGAGAAGCT CACCCACAAG GAAGTGGATG 361 ATCTCTTCAG GGAAGCAGAT ATCGAACCCA ATGGCAAAGT GAAGTATGAT GAATTTATCC 421 ACAAGATCAC CCTTCCTGGA CGGGACTATT GCCCAACTTT CTTGTACAAA GTGGTTGATA 481 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 541 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGTTT 601 TCACAATCCA CCCCTAGAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 661 tgaaagatt